Original articles


1 ) Suzuki A, Yamasaki T, Hasebe R, Horiuchi M. Enhancement of binding avidity by bivalent binding enables PrPSc-specific detection by anti-PrP monoclonal antibody 132. PLoS One 14(6), e0217944, 2019. (doi: 10.1371/journal.pone.0217944)


2 ) Sawada K, Suzuki A, Yamasaki T, Iwamaru Y, Maatsuura Y, Miyazawa K, Masujin K, Atarashi R, Horiuchi M. Estimation of prion infectivity in tissues of cattle infected with atypical BSE by real time-quaking induced conversion assay. J Vet Med Sci 81(6), 846-850, 2019. (doi: 10.1292/jvms.19-0003)


3 ) Hagiwara K, Sato Y, Yamakawa Y, Hara H, Tobiume M, Okemoto-Nakamura Y, Sata T, Horiuchi M, Shibata H, Ono F. Tracking and clarifying differential traits of classical-and atypical L-type bovine spongiform encephalopathy prions after transmission from cattle to cynomolgus monkeys. PLoS One 14(5), e0216807, 2019. (doi: 10.1371/journal.pone.0216807)


4 ) Temuujin U, Tserendorj A, Fujiki J, Sakoda Y, Tseren-Ochir E, Okamatsu M, Matsuno K, Sharav T, Horiuchi M, Umemura T, Chultemdorj T. The first isolation and identification of canine parvovirus (CPV) type 2c variants during 2016-2018 genetic surveillance of dogs in Mongolia. Infect Genet Evol 73, 269-275, 2019. (doi: 10.1016/j.meegid.2019.05.006)


5 ) Muramatsu Y, Haraya N, Horie K, Uchida L, Kooriyama T, Suzuki A, Horiuchi M. Bergeyella zoohelcum isolated from oral cavities of therapy dogs. Zoonoses Public Health 66(8), 936-942, 2019. (doi: 10.1111/zph.12644)


6 ) Sakaguchi S, Shintani S, Kamio K, Sekiya A, Kato S, Muroi Y, Horiuchi M, Furuoka H. Selective neuronal vulnerability is involved in cerebellar lesions of Guinea pigs infected with bovine spongiform encephalopathy (BSE) prions: Immunohistochemical and electron microscopic investigations. Neuropathology 40(2), 167-179, 2019. (doi: 10.1111/neup.12613)



Original articles


1 ) Yamasaki T, Suzuki A, Hasebe R, Horiuch M. Flow cytometric detection of PrPSc in neurons and glial cells from prion-infected mouse brains. J Virol 92(1), e011457-17, 2018. (doi: 10.1128/JVI.01457-17)


2 ) Yamasaki T, Suzuki A, Hasebe R, Horiuch M. Retrograde transport by clathrin-coated vesicles is involved in intracellular transport of PrPSc in persistently prion-infected cells. Sci Rep 8(1), 12241, 2018. (doi: 10.1038/s41598-018-30775-1)



Original articles


1 ) Hasebe R, Nakao R, Ohnuma A, Yamasaki T, Sawa H, Takai S, Horiuchi M. Listeria monocytogenes serotype 4b strains replicate in monocytes/macrophages more than the other serotypes. J Vet Med Sci 79, 962-969, 2017. (doi: 10.1292/jvms.16-0575)


2 ) Shan Z, Hirai Y, Hayashi R, Yamasaki T, Hasebe R, Song CH, Horiuchi M. Therapeutic effect of autologous compact bone-derived mesenchymal stem cell transplantation on prion disease. J Gen Virol 98, 2615-2627, 2017. (doi: 10.1099/jgv.0.000907)


3 ) Yamasaki T, Suzuki A, Hasebe R, Horiuchi M. Flow Cytometric Detection of PrPSc in Neurons and Glial Cells from Prion-Infected Mouse Brains. J Virol 92, e01457-17, 2017. (doi: 10.1128/JVI.01457-17)



Original articles


1 ) Saijo E, Hughson AG, Raymond GJ, Suzuki A, Horiuchi M, Caughey B. PrPSc-Specific Antibody Reveals C-Terminal Conformational Differences between Prion Strains. J Virol 90, 4905-4913, 2017. (doi: 10.1128/JVI.00088-16)


2 ) Hasebe R, Tanaka M, Suzuki A, Yamasaki T, Horiuchi M. Complement factors alter the amount of PrP(Sc) in primary-cultured mouse cortical neurons associated with increased membrane permeability. Virology 496, 9-20, 2017. (doi: 10.1016/j.virol.2016.05.014)


3 ) Tanaka M, Fujiwara A, Suzuki A, Yamasaki T, Hasebe R, Masujin K, Horiuchi M. Comparison of abnormal isoform of prion protein in prion-infected cell lines and primary-cultured neurons by PrPSc-specific immunostaining. J Gen Virol 97, 2030-2042, 2017. (doi: 10.1099/jgv.0.000514)


4 ) Shan Z, Yamasaki T, Suzuki A, Hasebe R, Horiuchi M. Establishment of a simple cell-based ELISA for the direct detection of abnormal isoform of prion protein from prion-infected cells without cell lysis and proteinase K treatment. Prion 10, 305-318, 2017. (doi: 10.1080/19336896.2016.1189053)



Original articles


1 ) Nagasawa, Y., Takahashi, Itani, W., Watanabe, H., Hidaka, Y., Morita, S., Suzuki, K., Watanabe, K., Ohwada, S., Kitazawa, H., Imamura, M.,  Yokoyama, T.,  Horiuchi, M., Sakaguchi, S.,  Mohri, S., Rose, M. T., Nochi, T. and  Aso, H. 2015. Prion Protein Binds to Aldolase A Produced by Bovine Intestinal M Cells Open Journal of Veterinary Medicine, 5: 43-60. (doi: 10.4236/ojvm.2015.53007)


2 ) Hasebe, R., Tanaka, M., Suzuki, A., Yamasaki, T. and Horiuchi, M. 2015. Complement factors alter the amount of PrPSc in primary-cultured mouse cortical neurons associated with increased membrane permeability. in press.


3 ) Kabuki, H., Hasebe, R., Yamasaki, T. Chung-Hyun Song and Horiuchi, M. 2015. CD11b-positive microglia-specific gene expression analysis for prion infection. in press.


4 ) Suzuki, A., Yamasaki, T.,  Hasebe, R. and Horiuchi, M. 2015. Analysis for binding kinetics of anti-PrP monoclonal mAb132: towards elucidation of Mechanism of the PrPSc-specific detection by mAb132. in press.


5 ) Shan, Z., Yamasaki, T., Suzuki, A., Hasebe, R. and Horiuchi, M. 2015. High throughput detection of PrPSc from prion-infected cells without PK treatment: Cell-based ELISA for novel screening method for anti-prion compounds. in press.


6 ) Kuroda, M., Yamasaki, T., Suzuki, A., Hasebe, R. and Horiuchi, M. 2015. Analysis of activation state of astrocytes with the progression of prion diseases. in press.



Original articles


1 ) Hasebe, R., Suzuki, A., Yamasaki, T. and Horiuchi, M. 2014. Temporary upregulation of anti-inflammatory cytokine Il-13 expression in the brains of CD14 deficient mice in the early stage of prion infection. Biochem. Biophys. Res. Commun., 434: 125-130. (doi: 10.1016/j.bbrc.2014.10.043)


2 ) Yamasaki, T., Suzuki, A., Hasebe, R. and Horiuchi, M. 2014. Comparison of the anti-prion mechanism of four different anti-prion compounds, anti-PrP monoclonal antibody 44B1, pentosan polysulfate, chlorpromazine, and U18666A, in prion-infected mouse neuroblastoma cells. PLoS One, 9: e106516. (doi: 10.1371/journal.pone.0106516)


3 ) Uchida, L., Heriyato, A., Thongchai, C., Hanh, T. T., Horiuchi, M., Ishihara, K., Tamura, Y. and Muramatsu, Y. 2014. Genetic diversity in the prion protein gene (PRNP) of domestic cattle and water buffaloes in Vietnam, Indonesia and ThailandJ. Vet. Med. Sci., 76: 1001-1008. (doi: 10.1292/jvms.13-0642)


4 ) Hirano, M., Yoshii, K., Sakai, M., Hasebe, R. and Kariwa, H. 2014. Tick-borne flaviviruses alter membrane structure and replicate in dendrites of primary mouse neuronal cultures. J. Gen. Virol., 85: 849-861. (doi: 10.1099/vir.0.061432-0)


5 ) Kobayashi, S., Orba, Y., Yamaguchi, H., Takahashi, K., Sasaki, M., Hasebe, R., Kimura, T. and Sawa, H. 2014. Autophagy inhibits viral genome replication and gene expression stages in West Nile virus infection. Virus Res., 191: 83-91. (doi: 10.1016/j.virusres.2014.07.016)


6 ) Yamasaki, T., Baron, G. S., Suzuki, A., Hasebe, R. and Horiuchi, M. 2014. Characterization of intracellular dynamics of inoculated PrP-res and newly generated PrP(Sc) during early stage prion infection in Neuro2a cells. Virology., 450-451: 324-335. (doi: 10.1016/j.virol.2013.11.007)


7 ) Makino, Y., Suzuki, T., Hasebe, R., Kimura, T., Maeda, A., Takahashi, H. and Sawa, H. 2014. Establishment of tracking system for West Nile virus entry and evidence of microtubule involvement in particle transport. J. Virol. Method., 195: 250-257. (doi: 10.1016/j.jviromet.2013.10.002)


Publications other than original articles


1 ) Horiuchi, M. 2014. Looking back on the occurrence of BSE and its control measures. J. Jpn. Vet. Med. Assoc., 67: 345-353. (in Japanese).



Original articles


1 ) Ohsawa, N., Song, C. -H., Suzuki, A., Furuoka, H., Hasebe, R. and Horiuchi, M. 2013. Therapeutic effect of peripheral administration of an anti-prion protein antibody on mice infected with prions. Microbiol. Immunol., 57: 288-297.


2 ) Sakai, K., Hasebe, R., Takahashi, Y., Song, C. -H., Suzuki, A., Yamasaki, T. and Horiuchi, M. 2013. Absence of CD14 delays progression of prion diseases accompanied by increased microglial activation. J. Virol., 87: 13433-13445.


Publications other than original articles


1 ) Yamasaki, T. and Horiuchi, M. 2013. Infection and intracellular dynamics of prions. Dementia Japan, 27: 215-224 (in Japanese).



Original articles


1 ) Hasebe, R., Raymond, G. J., Horiuchi, M. and Caughey, B. 2012. Reaction of complement factors varies with prion strains in vitro and in vivoVirology, 423: 205-213.

2) Yamasaki, T., Suzuki, A., Shimizu, T., Watarai, M., Hasebe, R. and Horiuchi, M. 2012. Characterization of intracellular localization of PrPSc in prion-infected cells using monoclonal antibody that recognizes the region consisting of amino acids 119-127 of mouse PrPJ. Gen. Virol., 93: 668-680.

3) Yoshikawa, Y., Horiuchi, M., Ishiguro, N., Kadohira, M., Kai, S., Mizusawa, H., Nagata, C., Onodera, T., Sata, T., Tsutsui, T., Yamada, M. and Yamamoto, S. 2012. Alternative BSE Risk Assessment Methodology of Imported Beef and Beef Offal to JapanJ. Vet. Med. Sci., 74: 959-968.

4) Nakato, G., Hase, K., Suzuki, M., Kimura, M., Ato, M., Tobiume, M., Horiuchi, M., Atarashi, R., Nishida, N., Watarai, M, Imaoka, K. and Ohno H. 2012. Brucella abortus exploits a cellular prion protein on intestinal M cells as an invasive receptorJ. Immunol., 189: 1540-1544.



Original articles


1 ) Sassa, Y., Yamamoto, H., Mochizuki, M., Umemura, T., Horiuchi, M., Ishiguro, N., and Miyazawa, T. 2011. Successive deaths of a captive snow leopard (Uncia uncia) and a serval (Leptailurus serval) by infection with feline panleukopenia virus at Sapporo Maruyama ZooJ. Vet. Med. Sci., 73: 491-494.

2 ) Furuoka, H., Horiuchi, M., Yamakawa, Y. and Sata, T. 2011. Predominant involvement of the cerebellum in guinea pigs infected with bovine spongiform encephalopathy (BSE)J. Comp. Pathol., 144: 269-276. 

3 ) Song C.-H., Honmou, O., Furuoka, H. and Horiuchi, M. 2011. Identification of chemoattractive factors involved in the migration of bone marrow-derived mesenchymal stem cells to brain lesions caused by prionsJ. Virol., 85: 11069-11078. 

4 ) Sasaki, M., Kim, E., Igarashi, M., Ito, K., Hasebe, R., Fukushi, H., Sawa, H. and Kimura, T. 2011. Single amino acid residue in the A2 domain of major histocompatibility complex class I is involved in the efficiency of equine herpesvirus-1 entryJ. Biol. Chem., 286: 39370-39378. 

5 ) Sasaki, M., Hasebe, R., Makino, Y., Suzuki, T., Fukushi, H., Okamoto, M., Matsuda, K., Taniyama, H., Sawa, H. and Kimura T. 2011. Equine major histocompatibility complex class I molecules act as entry receptors that bind to equine herpesvirus-1 glycoprotein D. Genes Cells, 16: 343-357. 


Publication other than original articles


1 ) Horiuchi, M. 2011. Prion Diseases. The Medical Frontline, 66: 2713-2720. (in Japanese)



Original articles


1 ) Nakamitsu, S., Kurokawa, A., Yamasaki, T., Uryu, M., Hasebe, R. and Horiuchi, M. 2010. Cell-density dependent increase of the amount of protease-resistant PrP in prion-infected Neuro2a mouse neuroblastoma cellsJ. Gen. Virol., 91: 563-569. (doi: 10.1099/vir.0.016287-0)

2 ) Sakata, H., Horiuchi, M., Takahashi, I. and Kinjo, M. 2010. Confomational Analysis of Soluble Oligomers of GFP Tagged Prion Protein by Fluorescence Fluctuation SpectroscopyCurr. Pharm. Biotechnol., 11: 87-95. (doi: 10.2174/138920110790725357) 

3 ) Sato, Y., Shimonohara, N., Hanaki, K.I., Goto, M., Yamakawa ,Y., Horiuchi, M., Takahashi, H., Sata, T. and Nakajima, N. 2010. ImmunoAT method: an initial assessment for the detection of abnormal isoforms of prion protein in formalin-fixed and paraffin-embedded tissuesJ. Virol. Methods., 165: 261-267. (doi:10.1016/j.jviromet.2010.02.006)

4 ) Watanabe, Y., Hiraoka, W., Igarashi, M., Ito, K., Shimoyama, Y., Horiuchi, M., Yamamori, T., Yasui, H., Kuwabara, M., Inagaki, F. and Inanami, O. 2010. A novel copper(II) coordination at His186 in full-length murine prion protein. Biochem. Biophys. ResCommun., 394: 522-528. (doi:10.1016/j.bbrc.2010.03.003)

5 ) Hasebe, R., Suzuki, T., Makino, Y., Igarashi, M., Yamanouchi, S., Maeda, A., Horiuchi, M., Sawa, H. and Kimura, T. 2010. Transcellular transport of West Nile virus-like particles across human endothelial cells depends on residues 156 and 159 of envelope proteinBMC Microbiol., 10: 165. (doi:10.1186/1471-2180-10-165)

6 ) Sassa, Y., Yamasaki, T., Horiuchi, M., Inoshima, Y. and Ishiguro, N. 2010. The effects of lysosomal and proteasomal inhibitors on abnormal forms of prion protein degradation in murine macrophagesMicrobiol. Immunol., 54: 763-768. (doi: 10.1111/j.1348-0421.2010.00272.x.)



Original articles


1 ) Zheng, K., Zhou, HQ., Yan, J., Ke, CW., Maeda, A., Maeda, J., Takashima, I., Kurane, I., Ma, H., and Xie, XM. 2009. Molecular characterization of the E gene of dengue virus type 1 isolated in Guangdong province, China, in 2006. Epidemiol. Infect., 137: 73-78. 

2 ) Shindoh, R., Kim, C.-L., Song, C.-H., Hasebe, R., and Horiuchi, M. 2009. The region approximately between amino acids 81 and 137 of proteinase K-resistant PrPSc is critical for the infectivity of the Chandler prion strainJ. Virol., 83: 3852-3860.

3 ) Song, C.-H., Honmou, O., Nakamura, K., Hamada, H., Furuoka, H., Hasebe, R., and Horiuchi, M. 2009. Effect of transplantation of immortalized human bone marrow-derived mesenchymal stem cells on mice infected with prionsJ. Virol., 83: 5918-5927. 

4 ) Maeda, A., Murata, R., Akiyama, M., Takashima, I., Kariwa, H., Watanabe, T., Kurane, I., and Maeda, J. 2009. A PCR-based protocol for the generation of a recombinant West Nile virusVirus Res., 144: 35-43. 

5 ) Hara, Y., Hasebe, R., Sunden, Y., Ochiai, K., Honda, E., Sakoda, Y., and Umemura, T. 2009. Propagation of swine hemagglutinating encephalomyelitis virus and pseudorabies virus in dorsal root ganglia cellsJ. Vet. Med. Sci., 71: 595-601. 

6 ) Hasebe, R., Sasaki, M., Sawa, H., Wada, R., Umemura, T., and Kimura T. 2009. Infectious entry of equine herpesvirus-1 into host cells through different endocytic pathwaysVirology, 393:198-209. 

7 ) Horiuchi, M., Karino, A., Furuoka, H., Ishiguro, N., Kimura, K., and Shinagawa, M. 2009. Generation of monoclonal antibody that distinguishes PrPSc from PrPC and neutralizes prion infectivity. Virology, 394: 200-207. 



Original articles


1 ) Watanabe, Y., Hiraoka, W., Shimoyama, Y., Horiuchi, M. and Kuwabara, M. 2008. Instability of familial spongiform encephalopathy-related prion mutants. Biochem. Biophys.Res. Commun, 366: 244-249.

2 ) Song, C.-H., Furuoka, H., Kim, C.-L., Ogino, M., Suzuki, A., Hasebe, R. and Horiuchi, M. 2008. Effect of intraventricular infusion of anti-prion protein monoclonal antibodies on disease progression in prion-infected miceJ. Gen. Virol, 89: 1533-1544.

3 ) Muramatsu, Y., Sakemi, Y., Horiuchi, M., Ogawa, T., Suzuki, K., Kanameda, M., Tran Thi Hanh, T. T. and Tamura, Y. 2008. Frequencies of PRNP gene polymorphisms in Vietnamese dairy cattle for potential association with BSE. Zoonoses Public Health, 55: 267-273.

4 ) Takada, N, Horiuchi, M., Sata, T. and Sawada. Y, 2008. Evaluation of methods for removing central nervous system tissue contamination from the surface of beef carcasses after splittingJ. Vet. Med. Sci, 70: 1225-1230.

5 ) Watanabe, K., Tachibana, M., Tanaka, S., Furuoka, H., Horiuchi, M., Suzuki, H. and Watarai, M. 2008. Heat shock cognate protein 70 contribute Brucella invasion into trophoblast giant cells that cacuse infectious abortionBMC Microbiol, 8: 212.

6 ) Maeda, A., Maeda, J., Takagi, H. and Kurane, I. 2008. Detection of small RNAs containing the 5'- and the 3'-end sequences of viral genome during West Nile virus replication. Virology, 371: 130-138.

7 ) Maeda, J., Takagi, H., Hashimoto, S., Kurane, I. and Maeda, A. 2008. A PCR-based protocol for generating West Nile virus replicons. J. Virol. Methods, 148: 244-252.

8 ) Nakauchi, M., Kariwa, H., Kon, Y., Yoshii, K., Maeda, A. and Takashima, I. 2008. Analysis of Severe Acute Respiratory Syndrome Coronavirus Structural Proteins in Virus-Like Particle Assembly. Microbiol. Immunol, 52: 625-630.

9 ) Abu Daud, N. H., Kariwa, H., Tkachenko, E., Dzagurnova T., Medvedvedkina, O., Tkachenko, P., Ishizika M., Seto, T., Miyashita D., Sanada T., Nakauchi, M., Yoshii, K., Meda A., Yoshimatsu, K. Arikawa, J. and Takashima I. 2008. Genetic and antigenetic analyses of a Puumala virus isolate as a potential vaccine strain. Jpn. J. Vet. Res, 56: 151-165.


Publications other than original articles


1 ) Horiuchi, M. 2008. Propagation of prion and its inhibition. Virus Infection Seminar, 10: 13-25. (in Japanese)

2 ) Hasebe, R. 2008. ABC for prion diseases. Small Animal Clinic, 152: 4-9. (in Japanese)



Original articles


1 ) Furuoka, H., Yabuzoe, A., Horiuchi, M., Tagawa, Y., Yokoyama, T., Yamakawa, Y., Shinagawa, M. and Sata, T. 2007. Speciesspecificity of a panel of prion protein antibodies for the immunohistochemical study of animal and human prion diseases. J. Comp. Pathol., 136: 9-17.

2 ) Uryu, M., Karino, A., Kamihara, Y. and Horiuchi, M. 2007. Characterization of prion susceptibility in Neuro2a mouse neuroblastoma cell subclones. Microbiol. Immunol., 51: 661-667.

3 ) Ohara, J., Tokari, T., Kurokawa, A., Maeda, J., Ishiguro, N., Furuoka, H. and Horiuchi, M. 2007. Frequencies of PrP genotypes in meat breeds of Japanese sheep and trail of selective breeding in experimental sheep flock. J. Vet. Med. Sci., 69: 1325-1329.

4 ) Fujii, F., Horiuchi, M., Ueno, M., Sakata, H., Nagao, I., Tamura, M. and Kinjo, M. 2007. Detection of prion protein immune complex for bovine spongiform encephalopathy diagnosis using fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy. Anal. Biochem., 370: 131-141.


Publications other than original articles


1 ) Horiuchi,M. 2007. Prion. Clinical Virology, 35: 293-300 (in Japanese)

2 ) Nakamitsu, S. and Horiuchi, M. 2007. Bovine spongiform encephalopathy. Clinical Virology, 35: 301-310 (in Japanese)

3 ) Horiuchi, M. and Nakamitsu, S. 2007. Prion diseases in animals-Bovine spongiform encephalopathy. Japanese Journal of Clinical Medicine, 65: 1513-1520 (in Japanese)



Original articles


1 ) Horiuchi, M., Furuoka, H., Kitamura, N. and Shinagawa, M. 2006. Alymphoplasia mice are resistant to prion infection via oral route. Jpn. J. Vet Med., 53: 150-159.

2 ) Nakamitsu, S., Miyazawa, T., Horiuchi, M., Onoe, S., Ohoba, Y., Kitagawa, H. and Ishiguro, N. 2006. Sequence variation of bovine prion protein gene in Japanese cattle (Holstein and Japanese Black). J. Vet. Med. Sci., 68: 27-33.

3 ) Iwata, N., Sato, Y., Higuchi, Y., Nohtomi, K., Nagata, N., Hasegawa, H., Tobiume, M., Nakamura, Y., Hasegawa, K., Furuoka, H., Horiuchi, M., Yamakawa, Y. and Sata, T. 2006. Distribution of PrPSc in cattle with bovine spongiform encephalopathy slaughtered at abattoirs in Japan. Jpn. J. Infect. Dis., 59: 100-107.

4 ) Yamaguchi, S., Nishida, Y., Sasaki, K., Kambara, M., Kim, C.‐L., Ishiguro, N., Nagatsuka T., Uzawa, H. and Horiuchi, M. 2006. Inhibition of PrPSc formation by synthetic O-sulfated glycopyranosides and their polymers. Biochem. Biophys. Res. Commun., 349: 485-491.

5 ) Watanabe, Y., Inanami, O., Horiuchi, M., Hiraoka, W., Shimoyama, Y., Inagaki, F. and Kuwabara, M. 2006. Identification of pH-sensitive regions in the mouse prion by the cysteine-scanning spin-labeling ESR technique. Biochem. Biophys. Res. Commun., 350: 549-556.


Publications other than original articles


1 ) Maeda, A., Maeda, J., and Kurane, I. 2006. SUMOylation in viral infection : Dose SUMO wressle against or help for viral infection. Curr. Top. Biochem., pp. 99-120. Eds. Ohyama T. et al.



Original articles


1 ) Furuoka, H., Yabuzoe, A., Horiuchi, M., Tagawa, Y., Yokoyama, T., Yamakawa, Y., Shinagawa, M. and Sata, T. 2005. Effective antigen-retrieval method for immunohistochemical detection of abnormal isoform of prion proteins in animals. Acta Neuropathol., 109: 263-271.

2 ) Kataoka, N., Nishimura, M., Horiuchi, M. and Ishiguro , N. 2005. Surveillance of chronic wasting disease in sika deer, Cervus nippon, from Tokachi district in Hokkaido. J. Vet. Med. Sci., 67: 349-351.

3 ) Kurosaki, Y., Ishiguro, N., Horiuchi, M. and Shinagawa, M. 2005. Polymorphisms of caprine PrP gene detected in Japan. J. Vet. Med. Sci., 67: 321-323.

4 ) Inanami, O., Hashida, S., Iizuka, D., Horiuchi, M., Hiraoka, W., Shimoyama, Y. , Nakamura, H.,Inagaki, F. and Kuwabara, M. 2005. Conformational change in fulllength mouse prion : A site-directed spinlabeling study. Biochem . Biophys. Res. Commun., 335: 785-792.

5 ) Morikawa, S., Sakiyama, T., Hasegawa, H., Saijo, M., Maeda, A., Kurane, I., Maeno, G., Kimura, J., Hirama, C., Yoshida, T., Asahi-Ozaki, Y., Sata, T., Kurata, T. and Kojima, A. 2005. An attenuated LC16m8 smallpox vaccine : Analysis of full-Genome sequence and induction of immune protection. J. Virol., 79: 11873-11891.

6 ) Saijo, M., Tang, Q., Shimayi, B., Han, L., Zhang, Y., Asiguma, M., Tianshu, D. Maeda, A., Kurane, I. and Morikawa, S. 2005. Recombinant nucleocapsidproteinbased serological diagnosis of Crimean-Congo Hemorragic Fever virus infection. J. Med. Virol., 75: 295-299.


Publications other than original articles


1 ) Horiuchi,M. 2005. Biosynthesis and transmission of abnormal prion protein. Membrane, 30: 78-83. (in Japanese).

2 ) Horiuchi, M. 2005. Development of BSE diagnosis methods and its current situation. Virus Report, 2: 20-27. (in Japanese).

3 ) Horiuchi, M. 2005. Prion diseases as zoonsis. Virus, 55: 45-55. (in Japanese).

4 ) Horiuchi, M. 2005. Prion diseases as infectous disease transmissible from animals to humas. Jpn. J. Clin. Med., 63: 2213-2220. (in Japanese).

5 ) Maeda, A. and Kurane, I. 2005. Nipah virus infection. In : Animal-derived infection-diagnosis and prevention , pp. 101-105, Kamiyama, T. and Yamada, A. eds., Shinkou Koueki, Tokyo. (in Japanese).

6 ) Maeda, A. and Kurane, I. 2005. Hendra virus infection . In : Animal-derived infection- diagnosis and prevention , pp. 110-114, Kamiyama, T. and Yamada, A. eds., Shinkou Koueki, Tokyo. (in Japanese).



Original articles


1 ) Kim, C-L., Umetani, A., Matsui, T., Ishiguro, N., Shinagawa, M., and Horiuchi, M. 2003. Antigenic characterization of an abnormal isoform of prion protein using a new diverse panel of monoclonal antibodies. Virology, 320: 41-52.


2 ) Kim, C-L., Karino, A., Ishiguro, N., Shinagawa, M., and Horiuchi, M. 2004. Cell surface retention of PrPC by anti-PrP antibody prevents protease-resistant PrP formation. J. Gen. Virol., 85: 3473-3482.


3 ) Gombojav, A., Ishiguro, N., Horiuchi, M., and Shinagawa, M. 2004. Unique amino acid polymorphisms of PrP genes in Mongolian sheep breeds. J. Vet. Med. Sci., 66: 1293-1295.


4 ) Niikura, M., Maeda, A., Ikegami, T., Saijo, M., Kurane, I., and Morikawa, S. 2004. Modification of endothelial cell functions by Hantaan virus infection: prolonged hyper-permeability induced by TNF-alpha of hantaan virus-infected endothelial cell monolayers. Arch. Virol., 149: 1279-1292.


5 ) Saijo, M., Tang, Q., Shimayi, B., Han, L., Zhang, Y., Asiguma, M., Tianshu, D., Maeda, A., Kurane, I., and Morikawa, S. 2004. Possible horizontal transmission of crimean-congo hemorrhagic Fever virus from a mother to her child. Jpn. J. Infect. Dis., 57: 55-57.