Original articles


1 ) Higashikawa K, Horiguchi S, Tarisawa M, Shibata Y, Ohkura K, Yasui H, Takeda H, Kuge Y. Preclinical investigation of potential use of thymidine phosphorylase-targeting tracer for diagnosis of nonalcoholic steatohepatitis. Nucl Med Biol 82-83, 25-32, 2020. (doi: 10.1016/j.nucmedbio.2019.12.006)


Publication other than original articles


1 ) Yasui H, Iizuka D, Hiraoka W, Kuwabara M, Matsuda A, Inanami O. Nucleoside analogs as a radiosensitizer modulating DNA repair, cell cycle checkpoints, and apoptosis. Nucleosides Nucleotides Nucleic Acids 39(1-3), 439-452, 2020. (doi: 10.1080/15257770.2019.1670839)



Original articles


1 ) Shimizu Y, Zhao S, Yasui H, Nishijima KI, Matsumoto H, Shiga T, Tamaki N, Ogawa M, Kuge, Y. A novel PET Prode “[18F]DiFA” accumulates in hypoxic region via glutathione conjugation following reductive metabolism. Mol Imaging Biol 21(1), 122-129, 2019. (doi: 10.1007/s11307-018-1214-y)


2 ) Yoneshiro T, Shin W, Machida K, Fukano K, Tsubota A, Chen Y, Yasui H, Inanami O, Okamatsu-Ogura Y, Kimura K. Differentiation of bone marrow-derived cells toward thermogenic adipocytes in white adipose tissue induced by the β3 adrenergic stimulation. FASEB J 33(4), 5196-5207, 2019. (doi: 10.1096/fj.201801757RR)


3 ) Bhilwade HN, Tatewaki N, Konishi T, Nishida M, Eitsuka T, Yasui H, Inanami O, Handa O, Naito Y, Ikekawa N, Nishida H. The adjuvant effect of squalene, an active ingredient of functional foods, on doxorubicin-treated allograft mice. Nutr Cancer 71(7), 1153-1164, 2019. (doi: 10.1080/01635581.2019.1597900)


4 ) Watanabe S, Shiga T, Hirata K, Magota K, Okamoto S, Toyonaga T, Higashikawa K, Yasui H, Kobayashi J, Nishijima KI, Iseki K, Matsumoto H, Kuge Y, Tamaki N. Biodistribution and radiation dosimetry of the novel hypoxia PET Probe [18F]DiFA and comparison with [18F]FMISO. EJNMMI Res 9(1), 60, 2019. (doi: 10.1186/s13550-019-0525-6)


5 ) Morita A, Gulay KCM, Onishi S, Shibata Y, Yasui H, Kobayashi A, Kimura T. High drug efflux pump capacity and low DNA damage response induce doxorubicin resistance in canine hemangiosarcoma cell lines. Res Vet Sci 127, 1-10, 2019. (doi: 10.1016/j.rvsc.2019.09.011)


6 ) Hashimoto M, Saito N, Ohta H, Yamamoto K, Tashiro A, Nakazawa K, Inanami O, Kitamura H. Inhibition of ubiquitin-specific protease 2 causes accumulation of reactive oxygen species, mitochondria dysfunction, and intracellular ATP decrement in C2C12 myoblasts. Physiol Rep 7(14), e14193, 2019. (doi: 10.14814/phy2.14193)


7 ) Bo T, Yamamori T, Yamamoto K, Fujimoto M, Yasui H, Inanami O. Mitochondrial fission promotes radiation-induced increase in intracellular Ca2+ level leading to mitotic catastrophe in mouse breast cancer EMT6 cells. Biochem Biophys Res Commun 522(1), 144-150, 2019. (doi: 10.1016/j.bbrc.2019.11.027)


8 ) Shibata Y, Yasui H, Higashikawa K, Miyamoto N, Kuge Y. Erastin, a ferroptosis-inducing agent, sensitized cancer cells to X-ray irradiation via glutathione starvation in vitro and in vivo. PLoS One 14(12), e0225931, 2019. (doi: 10.1371/journal.pone.0225931)



Original articles


1) Yasui H, Kubota N, Nishizumi J, Sakai Y, Yamamori T, Inanami O. Preclinical study on hypoxic radiosensitizing effects of glycididazole in comparison with those of doranidazole in vitro and in vivo. Oncol Lett 15(2), 1993-1998, 2018. (doi: 10.3892/ol.2017.7481)


2 ) Nagane M, Kuppusamy ML, An J, Mast JM, Gogna R, Yasui H, Yamamori T, Inanami O, Kuppusamy P. Ataxia-telangiectasia mutated (ATM) kinase regulates eNOS expression and modulates radiosensitivity in endothelial cells exposed to ionizing radiation. Radiat Res 189(5), 519-528, 2018. (doi: 10.1667/RR14781.1)


3 ) Yamamoto K, Ikenaka Y, Ichise T, Bo T, Ishizuka M, Yasui H, Hiraoka W, Yamamori T, Inanami O. Evaluation of mitochondrial redox status and energy metabolism of X-irradiated HeLa cells by LC/UV, LC/MS/MS and ESR. Free Radic Res 52(6), 648-660, 2018. (doi: 10.1080/10715762.2018.1460472)


4 ) Yamamoto K, Yasui H, Bo T, Yamamori T, Hiraoka W, Yamazaki T, Yamada KI, Inanami O. Genotoxic responses of mitochondrial oxygen consumption rate and mitochondrial semiquinone radicals in tumor cells. Appl Magn Reson 49(8), 837-851, 2018. (doi: 10.1007/s00723-018-1007-0)


5 ) Tomita M, Yasui H, Higashikawa K, Nakajima K, Takakura H, Shiga T, Kuge Y, Ogawa M. Anti PD-1 treatment increases [18F]FDG uptake by cancer cells in a mouse B16F10 melanoma model. EJNMMI Res 8(1), 82, 2018. (doi: 10.1186/s13550-018-0433-1)


6 ) Komarov DA, Ichikawa Y, Yamamoto K, Stewart NJ, Matsumoto S, Yasui H, Kirilyuk IA, Khramtsov VV, Inanami O Hirata H. In vivo extracellular pH mapping of tumors using electron paramagnetic resonance. Anal Chem 90(23), 13938-13945, 2018. (doi: 10.1021/acs.analchem.8b03328)


7 ) Bo T, Yamamori T, Suzuki M, Sakai Y, Yamamoto K, Inanami O. Calmodulin-dependent protein kinase II (CaMKII) mediates radiation-induced mitochondrial fission by regulating the phosphorylation of dynamin-related protein 1 (Drp1) at serine 616. Biochem Biophys Res Commun 495(2), 1601-1607, 2018. (doi: 10.1016/j.bbrc.2017.12.012)


8 ) Sakai Y, Yamamori T, Yoshikawa Y, Bo T, Suzuki M, Yamamoto K, Ago T, Inanami O. NADPH oxidase 4 mediates ROS production in radiation-induced senescent cells and promotes migration of inflammatory cells. Free Radic Res 52(1), 92-102, 2018. (doi: 10.1080/10715762.2017.1416112)


9 ) Shino H, Otsuka-Yamasaki Y, Sato T, Ooi K, Inanami O, Sato R, Yamasaki M. Familial congenital methemoglobinemia in pomeranian dogs caused by a missense variant in the NADH-cytochrome b5 reductase gene. J Vet Intern Med 32(1), 165-171, 2018. (doi: 10.1111/jvim.15031)



Original articles


1 ) Kubota H, Komarov DA, Yasui H, Matsumoto S, Inanami O, Kirilyuk IA, Khramtsov W, Hirata H. Feasibility of in vivo three-dimensional T 2* mapping using dicarboxy-PROXYL and CW-EPR-based single-point imaging. MAGMA. 6, 291-298, 2017. (doi: 10.1007/s10334-016-0606-8)


2 ) Kitaya T, Maeda T, Yasui H, Mizukawa H, Inanami O, Nakata A, Miura T, Hosokawa Y,Kuwabara M. Attempts of Radiation Dose Measurement in the Teeth of Mice Living around the Nuclear Power Plant in Fukushima Using Electron Spin Resonance Spectroscopy. Radiation Environment and Medicine 6, 1-5, 2017


3 ) Yasui H, Yamamoto K, Suzuki M, Sakai Y, Bo T, Nagane M, Nishimura E, Yamamori T,Yamasaki T,Yamada KI, Inanami O. Lipophilic triphenylphosphonium derivatives enhance radiation-induced cell killing via inhibition of mitochondrial energy metabolism in tumor cells. Cancer lett 390, 160-167, 2017. (doi: 10.1016/j.canlet.2017.01.006)


4 ) Sun L, Moritake T, Ito K, Matsumoto Y, Yasui H, Nakagawa H, Hirayama A, Inanami O, Tsuboi K. Metabolic analysis of radioresistant medulloblastoma stem-like clones and potential therapeutic targets. PLoS One 12, e0176162, 2017. (doi: 10.1371/journal.pone.0176162)


5 ) Suzuki M, Yamamori T, Bo T, Sakai Y, Inanami O. MK-8776, a novel Chk1 inhibitor, exhibits an improved radiosensitizing effect compared to UCN-01 by exacerbating radiation-induced aberrant mitosis. Transl Oncol 10, 491-500, 2017. (doi: 10.1016/j.tranon.2017.04.002)


6 ) Amida T, Nakaoka R, Komarov DA, Yamamoto K, Inanami O, Matsumoto S, Hirata H. A 750-MHz electronically tunable resonator using microstrip line couplers for electron paramagnetic resonance imaging of a mouse tumor-bearing leg. IEEE Trans Biomed Eng 65, 1124 - 1132, 2017. (doi: 10.1109/TBME.2017.2743232)


7 ) Yasui H, Kawai T, Matsumoto S, Saito K, Devasahayam N, Mitchell JB, Camphausen K, Inanami O, Krishna MC. Quantitative imaging of pO2 in orthotopic murine gliomas: hypoxia correlates with resistance to radiation. Free Radic Res 51, 861-871, 2017.(doi: 10.1080/10715762.2017.1388506)


8 ) Yamasaki M, Watanabe N, Yamamori T, Otsuguro K, Uchida N, Iguchi A, Ohta H, Takiguchi M. Intracellular diminazene aceturate content and adenosine incorporation in diminazene aceturate-resistant Babesia gibsoni isolate in vitro. Exp Parasitol 183, 92-98, 2017. (doi: 10.1016/j.exppara.2017.10.016)


Publications other than original articles


1 ) Masaki N, Hironobu Y, Tohru Y, Periannan K, Osamu I. Radiation-induced nitric oxide production in endothelial cells re-oxygenates solid tumor. Radiation Biology Research Communications 52, 173-182, 2017. (in Japanese) 



Original articles


1 ) Maeda K, Yasui H, Matsuura T, Yamamori T, Suzuki M, Nagane M, Nam JM, Inanami O, Shirato H. Evaluation of the relative biological effectiveness of spot-scanning proton irradiation in vitro. J Radiat Res 57, 307-311, 2016. (doi : 10.1093/jrr/rrv101)


2 ) Suzuki M, Yamamori T, Yasui H, Inanami O. Effect of MPS1 Inhibition on Genotoxic Stress Responses in Murine Tumour Cells. Anticancer Res 36, 2783-2792, 2016.


3 ) Maeda K, Yasui H, Yamamori T, Matsuura T, Takao S, Suzuki M, Matsuda A, Inanami O, Shirato H. A Nucleoside Anticancer Drug, 1-(3-C-Ethynyl-β-D-Ribo Pentofuranosyl) Cytosine, Induces Depth-Dependent Enhancement of Tumor Cell Death in Spread-Out Bragg Peak (SOBP) of Proton Beam. PLoS One 11, e0166848, 2016. (doi: 10.1371/journal.pone.0166848)


4 ) Yamamori T , Sasagawa T, Ichii O, Hiyoshi M,Bo T, Yasui H, Kon Y, Inanami O. Analysis of the mechanism of radiation-induced upregulation of mitochondrial abundance in mouse fibroblasts. J Radiat Res 58, 292–301, 2016. (doi : 10.1093/jrr/rrw113)



Original articles


1 ) Sakai, Y., Yamamori, T., Yasui H. and Inanami, O. 2015. Downregulation of the DNA repair enzyme apurinic/apyrimidinic endonuclease 1 stimulates transforming growth factor-β1 production and promotes actin rearrangement. Biochem. Biophys. Res. Commun. 461: 35-41. (doi: 10.1016/j.bbrc.2015.03.163)


2 ) Sakata, K., Kondo, T., Mizuno, N., Shoji, M., Yasui, H., Yamamori, T., Inanami, O., Yokoo, H., Yoshimura, N., Hattori, Y. 2015. Roles of ROS and PKC-βⅡin ionizing radiarion-induced eNOS activation in human vascular endothelial cells. Vascul. Pharmacol., 70: 55-65. (doi: 10.1016/j.vph.2015.03.016)



Original articles


1 ) Yasui, H., Takeuchi, R., Nagane, M., Meike, S., Nakamura, Y., Yamamori, T., Ikenaka, Y., Kon, Y., Murotani, H., Oishi, M., Nagasaki, Y. and Inanami O. 2014. Radiosensitization of tumor cells through endoplasmic reticulum stress induced by PEGylated nanogel containing gold nanoparticles. Cancer Lett., 347: 151-158. (doi: 10.1016/j.canlet.2014.02.005)


2 ) Goodwin, J., Yachi, K., Nagane, M., Yasui, H., Miyake, Y., Inanami, O., Bobko, A. A., Khramtsov, V. V. and Hirata, H. 2014. In vivo tumour extracellular pH monitoring using electron paramagnetic resonance: the effect of X-ray irradiation. NMR Biomed., 27: 453-458. (doi: 10.1002/nbm.3081)


3 ) Nishida, N., Yasui, H., Nagane, M., Yamamori, T. and Inanami, O. 2014. 3-Methyl pyruvate enhances radiosensitivity through increasing mitochondria-derived reactive oxygen species in tumor cell lines. J. Radiat. Res., 55: 455-463. (doi: 10.1093/jrr/rrt142)


Review article


1 ) Kuwabara, M., Hiraoka, W. and Inanami, O. 2014. Chapter 9. Applications of the spin-trapping method in radiation biology, In Applications of EPR in Radiation Research, pp. 353-384, Lund, A., Shiotani, M., eds., Springer International Publishing. (doi: 10.1007/978-3-319-09216-4_9)



Original articles


1 ) Yamamori, T., Meike, S., Nagane, M., Yasui, H. and Inanami, O. 2013. ER stress suppresses DNA double-strand break repair and sensitizes tumor cells to ionizing radiation by stimulating proteasomal degradation of Rad51. FEBS Lett., 587: 3348-3353.


2 ) Nagane, M., Yasui, H., Yamamori, T., Zhao, S., Kuge, Y., Tamaki, N., Kameya, H., Nakamura, H., Fujii, H. and Inanami, O. 2013. Radiationinduced nitric oxide mitigates tumor hypoxia and radioresistance in a murine SCCVII tumor model. Biochem. Biophys. Res. Commun., 437: 420-425.


3 ) Yasui, H., Asanuma, T., Kino, J., Yamamori, T., Meike, S., Nagane, M., Kubota, N., Kuwabara, M. and Inanami, O. 2013. The prospective application of a hypoxic radiosensitizer, doranidazole to rat intracranial glioblastoma with blood brain barrier disruption. BMC Cancer 13: 106 (9 pages).


4 ) Matsumoto, S., Saito, K., Yasui, H., Morris, H. D., Munasinghe, J. P., Lizak, M., Merkle, H., Ardenkjaer-Larsen, J. H., Choudhuri, R., Devasahayam, N., Subramanian, S., Koretsky, A. P., Mitchell, J. B. and Krishna, M. C. 2013. EPR oxygen imaging and hyperpolarized 13C MRI of pyruvate metabolism as noninvasive biomarkers of tumor treatment response to a glycolysis inhibitor 3-bromopyruvate. Magn. Reson. Med. 69: 1443-1450.


5 ) Jung, S. B., Kim, C. S., Kim, Y. R., Naqvi, A., Yamamori, T., Kumar, S., Kumar, A. and Irani, K. 2013. Redox factor-1 activates endothelial SIRTUIN1 through reduction of conserved cysteine sulfhydryls in its deacetylase domain. PLoS One 8: e65415 (11 pages).



Original articles


1 ) Eitaki, M., Yamamori, T., Meike, S., Yasui, H. and Inanami, O. 2012. Vincristine enhances amoeboid-like motility via GEF-H1/RhoA/ROCK/Myosin light chain signaling in MKN45 cellsBMC Cancer12: 469.

2 ) Indo, H. P., Inanami, O., Koumura, T., Suenaga, S., Yen, H. C., Kakinuma, S., Matsumoto, K., Nakanishi, I., St, Clair. W., St, Clair. D. K., Matsui, H., Cornette, R., Gusev, O., Okuda, T., Nakagawa, Y., Ozawa, T. and Majima, H. J. 2012. Roles of mitochondria-generated reactive oxygen species on X-ray-induced apoptosis in a human hepatocellular carcinoma cell line, HLEFree Radic. Res.46: 1029-1043.

3 ) Yamamori, T., Yasui, H., Yamazumi, M., Wada, Y., Nakamura, Y., Nakamura, H. and Inanami, O. 2012. Ionizing radiation induces mitochondrial reactive oxygen species production accompanied by upregulation of mitochondrial electron transport chain function and mitochondrial content under control of the cell cycle checkpoint. Free Radic. Biol. Med.53: 260-270.

4 ) Saito, K., Matsumoto, S., Yasui, H., Devasahayam, N., Subramanian, S., Munasinghe, J. P., Patel, V., Gutkind, J. S., Mitchell, J. B. and Krishna, M. C. 2012. Longitudinal imaging studies of tumor microenvironment in mice treated with the mTOR inhibitor rapamycin. PLoS ONE7: e49456.

5 ) Krishna, M. C., Matsumoto, S., Yasui, H., Saito, K., Devasahayam, N., Subramanian, S. and Mitchell, J. B. 2012. Electron paramagnetic resonance imaging of tumor pO2. Radiat. Res.177: 376-386.



Original articles


1 ) Yamamori, T., Mizobata, A., Saito, Y., Urano, Y., Inanami, O., Irani, K. and Noguchi, N. 2011. Phosphorylation of p66shc mediates 6-hydroxydopamine cytotoxicityFree Radic. Res., 45: 342-350. (doi:10.3109/10715762.2010.532496) 

2 ) Meike, S., Yamamori, T., Yasui, H., Eitaki, M., Matsuda, A. and Inanami, O. 2011. 8-amino-adenosine Enhances Radiation-induced Cell Death in Human Lung Carcinoma A549 CellsJ. Radiat. Res., 52: 456-463. (doi: 10.1269/jrr.10188)

3 ) Meike, S., Yamamori, T., Yasui, H., Eitaki, M., Matsuda, A., Morimatsu, M., Fukushima, M., Yamasaki, Y. and Inanami, O. 2011. A nucleoside anticancer drug, 1-(3-C-ethynyl-β-D-ribo-pentofuranosyl)cytosine (TAS106), sensitizes cells to radiation by suppressing BRCA2 expression. Mol. Cancer10: 92. (doi: 10.1186/1476-4598-10-92) 

4 ) Kobayashi, S., Abe,  Y,. Inanami, O., Oda, S., Yamauchi, K., Hankanga, C., Yasuda, J. and Sato, R. 2011. Oral administration of bovine lactoferrin upregulates neutrophil functions in a dog with familial β2-integrin-related neutrophil dysfunction. Vet. Immunol. Immunopathol., 143: 155-161. (doi:10.1016/j.vetimm.2011.05.027) 

5 ) Matsumoto, S., Batra, S., Saito, K., Yasui, H., Choudhuri, R., Gadisetti, C., Subramanian , S., Devasahayam, N., Munasinghe, J.P., Mitchell, J.B. and Krishna, M.C. 2011. Antiangiogenic agent sunitinib transiently increases tumor oxygenation and suppresses cycling hypoxia. Cancer Res., 71: 6350-6359. (doi: 10.1158/0008-5472.CAN-11-2025) 

6 ) Yamanaka, K., Saito, Y., Yamamori, T., Urano, Y. and Noguchi, N. 24(S)-hydroxycholesterol induces neuronal cell death through necroptosis, a form of programmed necrosis. J. Biol. Chem., 286: 24666-24673. (doi:10.1074/jbc.M111.236273) 


Publications other than articles


1 ) Yamamori, T. and Irani, K. 2011. Deacetylation of AP endonuclease 1 by Sirt1 regulates DNA base excision repair activity. Biomedical Gerontology35: 29-32. (in Japanese) 

2 )Yamamori, T. and Irani, K. 2011. Regulation of DNA repair enzyme AP endonuclease 1 by lifespan regulation-related protein Sirt1. The Journal of the Hokkaido Veterinary Medical Association55: 45-49. (in Japanese) 



Original articles


1 ) Yamamori, T., DeRicco, J., Naqvi, A., Hoffman, T. A., Mattagajasingh, I., Kasuno, K., Jung SB, Kim C. S. and Irani, K. 2010. SIRT1 deacetylates APE1 and regulates cellular base excision repairNucleic Acids Res., 38: 832-845. (doi: 10.1093/nar/gkp1039)

2 ) Asanuma, T., Ono, M., Kubota, K., Hirose, A., Hayashi, Y., Saibara, T., Inanami, O., Ogawa, Y., Enzan, H., Onishi, S., Kuwabara, M. and Oben, J. A. 2010. Super paramagnetic iron oxide MRI shows defective Kupffer cell uptake function in non-alcoholic fatty liver diseaseGut, 59: 258-266. (doi:10.1136/gut.2009.176651)

3 ) Yasui, H., Ito, N., Yamamori, T., Nakamura, H., Okano, J., Asanuma, T., Nakajima, T., Kuwabara, M. and Inanami, O. 2010. Induction of neurite outgrowth by alpha-phenyl-N-tert-butylnitrone through nitric oxide release and Ras-ERK pathway in PC12 cellsFree Radic. Res., 44: 645-654. (doi:10.3109/10715761003692537) 

4 ) Watanabe, Y., Hiraoka, W., Igarashi, M., Ito, K., Shimoyama, Y., Horiuchi, M., Yamamori, T., Yasui, H., Kuwabara, M., Inagaki, F. and Inanami, O. 2010. A novel copper(II) coordination at His186 in full-length murine prion proteinBiochem. Biophys. Res. Commun., 394: 522-528. (doi: 10.1016/j.bbrc.2010.03.003)

5 ) Jung, S. B., Kim, C. S., Naqvi, A., Yamamori, T., Mattagajasingh, I., Hoffman, T. A., Cole, M. P., Kumar, A., DeRicco, J. S., Jeon, B. H. and Irani, K. 2010. Histone deacetylase 3 antagonizes aspirin-stimulated endothelial nitric oxide production by reversing aspirin-induced lysine acetylation of endothelial nitric oxide synthaseCirc. Res. 107: 877-887. (doi: 10.1161/CIRCRESAHA.110.222968)

6 ) Naqvi, A., Hoffman, T. A., DeRicco, J., Kumar, A., Kim, C. S., Jung, S. B., Yamamori, T., Kim, Y. R., Mehdi, F., Kumar, S., Rankinen, T., Ravussin, E. and Irani, K. 2010. A single-nucleotide variation in a p53-binding site affects nutrient-sensitive human SIRT1 expressionHum. Mol. Genet., 19: 4123-4133. (doi: 10.1093/hmg/ddq331)

7 ) Yasui, H., Matsumoto, S., Devasahayam, N., Munasinghe, J. P., Choudhuri, R., Saito, K., Subramanian, S., Mitchell, J. B., and Krishna, M. C. 2010. Low-field magnetic resonance imaging to visualize chronic and cycling hypoxia in tumor-bearing miceCancer Res., 70: 6427-6436. (doi: 10.1158/0008-5472.CAN-10-1350)

8 ) Matsumoto, S., Yasui, H., Mitchell, J. B. and Krishna, M. C. 2010. Imaging cycling tumor hypoxia. Cancer Res., 70: 10019-10023. (doi: 10.1158/0008-5472.CAN-10-2821) 


Publications other than articles


1 ) Yasui, H., Yamamori. T., Meike, S., Eitaki, M., Iizuka, D., Kuwabara, M. and Inanami, O. 2010. New insight into hypoxia-targeting cancer therapyRadiation Biology Research Communications45: 45-57. (in Japanese) 

2 ) Yasui, H. 2010. Report of overseas research experience, Spin Science14: 51-53. (in Japanese) 



Original articles


1 ) Kobayashi, S., Sato, R., Abe, Y., Inanami, O., Yasui, H., Omoe, K., Yasuda, J., Hankanga, C., Oda, S. and Sasaki, J. 2009. Canine neutrophil dysfunction caused by downregulation of β2-integrin expression without mutation. Vet. Immunol. Immunopathol., 130:187-196. 

2 ) Ogura, A., Oowada, S., Kon, Y., Hirayama, A., Yasui, H., Meike, S., Kobayashi, S., Kuwabara, M. and Inanami, O. 2009. Redox regulation in radiation-induced cytochrome c release from mitochondria of human lung carcinoma A549 cells.Cancer Lett.277:64-71.

3 ) Matsumoto, S., Yasui, H., Batra, S., Kinoshita, Y., Bernardo, M., Munasinghe, J., Utsumi, H., Choudhuri, R., Devasahayam, N., Subramanian, S., Mitchell, J.B. and Krishna, M.C. 2009. Simultaneous imaging of tumor oxygenation and microvascular permeability using Overhauser enhanced MRI., Proc. Natl. Acad. Sci. U.S.A.106: 17898-17903.


Publication other than articles


1 ) Kuwabara, M. 2009. Spin trapping (4)Spin Science12: 50-54. (in Japanese) 



Original articles


1 ) Ogura, A., Watanabe, Y., Iizuka, D., Yasui, H., Amitani, M., Kobayashi, S., Kuwabara, M., and Inanami, O. 2008. Radiation-induced apoptosis of tumor cells is facilitated by inhibition of the interaction between Survivin and Smac/DIABLO. Cancer Lett,259: 71-81.

2 ) Yasui, H., Ogura, A., Asanuma, T., Matsuda, A., Kashiwakura, I., Kuwabara, M., Inanami, O. 2008. Inhibition of HIF-1alpha by the anticancer drug TAS106 enhances X-ray-induced apoptosis in vitro and in vivo. Br. J. Cancer299: 1442-1452.

3 ) Asanuma, T., Yasui, H., Sato, M., Inanami, O. and Kuwabara, M. 2008. A BOLD-fMRI study of cerebral activation induced by injection of algesic chemical substances into the anesthetized rat forepawJpn. J. Vet. Res56: 99-107.

4) Kuwabara, M., Asanuma, T., Niwa, K. and Inanami, O. 2008. Regulation of cell survival and death signals induced by oxidative stress. J. Clin. Biochem. Nutr43: 51-57.

5 ) Chiang. Y. W., Otoshima, Y., Watanabe, Y., Inanami, O., Shimoyama, Y. 2008. Dynamics and local ordering of spin-labeled prion protein: an ESR simulationstudy of a highly pH-sensitive site. J. Biomol. Struct. Dyn26: 355-366.

6 ) Asanuma, T., Doblas, S., Tesiram, Y. A., Saunders, D., Cranford, R., Yasui, H., Inanami. O., Smith, N., Floyd, R. A., Kotake, Y. and Towner, R. A. 2008. Visualization of the protective ability of a free radical trapping compound against rat C6 and F98 gliomas with diffusion tensor fiber tractography. J. Magn. Reson. Imaging28: 574-587.

7 ) Asanuma, T., Doblas, S., Tesiram, Y. A., Saunders, D., Cranford, R., Pearson, J., Abbott, A., Smith, N. and Towner, R. A. 2008. Diffusion tensor imaging and fiber tractography of C6 rat glioma. J. Magn. Reson. Imaging28: 566-573.

8 ) Iizuka, D., Ogura, A., Kuwabara, M. and Inanami, O. 2008. Purvalanol A induces apoptosis and downregulation of antiapoptotic proteins through abrogation of phosphorylation of JAK2/STAT3 and RNA polymerase II. Anticancer Drugs19: 565-572.

9 ) Yamada, Y., Sato, R., Kobayashi, S., Hankanga, C., Inanami, O., Kuwabara, M.,  Momota, Y., Tomizawa, N. and Yasuda, J., 2008. The antiproliferative effect of bovine lactoferrin on canine mammary gland tumor cellsJ. Vet. Med. Sci70: 443-448.

10 ) Kobayashi, S., Sato, R., Aoki, T., Omoe, K., Inanami, O., Hankanga, C., Yamada, Y., Tomizawa, N., Yasuda, J. and Sasaki, J. 2008. Effect of bovine lactoferrin on functions of activated feline peripheral blood mononuclear cells during chronic feline immunodeficiency virus infectionJ. Vet. Med. Sci70: 429-435.

11) Takahashi, M., Yasui, H., Ogura, A., Asanuma, T., Kubota, N., Tsujitani, M., Kuwabara. M. and Inanami, O. 2008. X irradiation combined with TNF alpha-related apoptosis-inducing ligand (TRAIL) reduces hypoxic regions of human gastric adenocarcinoma xenografts in SCID miceJ. Radiat. Res49: 153-161.

12) Kamikawa, A., Ishii, T., Shimada, K., Makondo, K., Inanami, O., Sakane, N., Yoshida, T., Saito, M. and Kimura, K. 2008. Proinsulin C-peptide abrogates type-1 diabetes-induced increase of renal endothelial nitric oxide synthase in ratsDiabetes Metab. Res. Rev24: 331-338.

13) Oriya, A., Takahashi, K., Inanami, O., Miura, T., Abe, Y., Kuwabara. M., Kashiwakura, I. 2008. Individual differences in the radiosensitivity of hematopoietic progenitor cells detected in steady-state human peripheral blood. J. Radiat. Res49: 113-121.

14) Watanabe, Y., Hiraoka, W., Shimoyama, Y., Horiuchi, M., Kuwabara, M. and Inanami, O. 2008. Instability of familial spongiform encephalopathy-related prion mutants. Biochem. Biophys. Res. Commun366: 244-249.

15) Kosugi, T., Kawahara, K., Tanaka, M., Watanabe, Y. and Inanami, O. 2008. Neuron is the primary target of Ca2+ paradox-type insult-induced cell injury in neuron/astrocyte co-culturesNeurochem. Int52: 887-896.


Publication other than articles


1 ) Kuwabara, M. 2008. Spin trapping (2)Spin Science10: 55-60. (in Japanese)

2 ) Kuwabara, M. 2008. Spin trapping (3)Spin Science11: 145-152. (in Japanese)

3 ) Ogura, A., Yasui, H., Kuwabara, M. and Inanami, O. 2008. Radiation/UV and Oxidative stress. In: Oxidative Stress in Medicine Yoshikawa, T., Naito, Y. and Toyokuni, S., eds., pp.175-182, Shindan-to-Chiryosha, Tokyo.



Original Articles


1 ) Inoue, Y., Asanuma, T., Smith, N., Saunders, D., Oblander, J., Kotake, Y., Floyd, R. A. and Towner, R. A. Modulation of Fas-FasL related apoptosis by PBN in the early phases of choline deficient diet-mediated hepatocarcinogenesis in rats. Free Radic Res., 41: 972-980. 66 Information

2 ) Iizuka, D., Inanami, O., Kashiwakura, I. and Kuwabara, M. Purvalanol A enhances cell killing by inhibiting up-regulation of CDC2 kinase activity in tumor cells irradiated with high doses of X rays. Radiat. Res., 167: 563-571.

3 ) Yasui, H., Inanami, O., Asanuma, T., Iizuka, D., Nakajima, T., Kon, Y., Matsuda, A. and Kuwabara, M. Treatment combining Xirradiation and a ribonucleoside anticancer drug, TAS106, effectively suppresses the growth of tumor cells transplanted in mice. Int. J. Radiat. Oncol. Biol. Phys., 68: 218-228.

4 ) Ahmed, M., Kimura, K., Soliman, M., Yamaji, D., Okamatsu-Ogura, Y., Makondo, K., Inanami, O. and Saito, M. Effects of leptin and tumor necrosis factor-alpha on degranulation and superoxide production of polymorphonuclear neutrophils from Holstein cows. J. Vet. Med. Sci., 69: 125-131.

5 ) Nagahata, H., Higuchi, H., Inanami, O. and Kuwabara, M. Costimulatory effects of complement receptor type 3 and Fc receptor for IgG (FcγR) on superoxide production and signal transduction in bovine neutrophils. J. Vet. Med. Sci., 69: 993-937.

6 ) Takahashi, M., Inanami, O., Kubota, N., Tsujitani, M., Yasui, H., Ogura, A. and Kuwabara, M. Enhancement of cell death by TNF alpha-related apoptosis-inducing ligand (TRAIL) in human lung carcinoma A549 cells exposed to x rays under hypoxia. J. Radiat. Res., 48: 461-468.

7 ) Asanuma, T., Yasui, H., Inanami, O., Waki, K., Takahashi, M., Iizuka, D., Uemura, T., Durand, G., Polidori, A., Kon, Y., Pucci, B. and Kuwabara, M. A new amphiphilic derivative, N-{[4-(lactobionamido)methyl]benzylidene}-1, 1-dimethyl-2-(octylsulfanyl ) ethylamine Noxide, has a protective effect against copperinduced fulminant hepatitis in Long-Evans Cinnamon rats at an extremely low concentration compared with itsoriginal form alphaphenyl-N-(tert-butyl)nitrone. Chem. Biodivers., 4: 2253-2267.

8 ) Yasui, H.,Asanuma, T.,Watanabe, Y.,Waki, K., Inanami, O. and Kuwabara, M. Oral administration of Antioxidant Biofactor (AOBTM) ameliorates ischemia/reperfusion-induced neuronal death in the gerbil. Biofactors, 29: 113-121.


Publication other than original articles


1 ) Iizuka, D., Inanami, O., Yasui, H., Ogura, A. and Kuwabara, M. 2007. Mechanisms for the radiation-induced cell death by ablogation of G2 checkpoint. Radiat. Biol. Res. Commun., 42: 444-455 (in Japanese).



Original Articles


1 ) Asanuma, T., Kawahara, T., Inanami, O., Nakao, M., Nakaya, K., Ito, A., Takiguchi, M., Hashimoto,A. and Kuwabara,M. 2006. Magnetic resonance imaging of alveolar echinococcosis experimentally induced in the rat lung. J. Vet. Med. Sci., 68: 15-20.

2 ) Niwa, K., Sakai, J., Karino, T.,Aonuma, H., Watanabe, T., Ohyama, T. , Inanami O. and Kuwabara, M. 2006. Reactive oxygen species mediate shear stress-induced fluid-phase endocytosis in vascular endothelial cells. Free Radic. Res., 40: 167-174.

3 ) Waki, K., Inanami, O., Yamamori, T., Nagahata, H., and Kuwabara, M. 2006. Involvement of protein kinase Cδ in the activation of NADPH oxidase and the phagocytosis of neutrophils.Free Radic. Res., 40: 359-367.

4 ) Takahashi, E., Inanami, O., Asanuma, T. and Kuwabara, M. 2006. Effects of ceramide inhibition on radiation-induced apoptosis in human leukemia MOLT‐4cells. J. Radiat. Res., 47: 19-25.

5 ) Hori, M., Asanuma, T., Inanami, O., Kuwabara, M., Harashima, H. and Kamiya, H. 2006. Effects of overexpression and antisense RNA expression of Orf17, a MutT-type enzyme. Biol. Pharmacol.Bull., 29: 1087-1091.

6 ) Mizutani, T., Fukushi, S., Iizuka, D., Inanami, O., Kuwabara, M., Takashima, H., Yanagawa, H., Saijo, M., Kurane, I. and Morikawa, S. 2006. Inhibition of cell proliferation by SARS-CoV infection in Vero E6cells. FEMS Immunol. Med. Microbiol., 46: 236-243.

7 ) Nakajima, T., Wakasa, T., Okuma, Y., Inanami, O., Nomura, Y., Kuwabara, M. and Kawahara, K. 2006. Dual inhibition of protein phosphatase-1/2A and calpain rescue nerve growth factor-differentiated PC12 cells from oxygen-glucose deprivation-induced cell death. J. Neurosci. Res., 83: 459-168.

8 ) Kashiwakura, I., Inanami, O., Abe, Y., Satoh, K., Takahashi, T. A. and Kuwabara, M. 2006. Regeneration of megakaryocytopoiesis and thromboboiesis in vitro from X-irradiated human hematopoietic stem cells. Radiat. Res., 116: 345-351.

9 ) Monzen, S., Mori, T., Takahashi, K., Abe, M., Inanami, O., Kuwabara, M. and Kashiwakura, I. 2006. The effects of (-)-epigallocatechin-3-gallate on the proliferation and differentiation of human megakaryocytic progenitor cells. J. Radiat. Res., 47: 213-220.

10) Watanabe, Y., Inanami, O., Horiuchi, M., Hiraoka, W., Shimoyama, Y., Inagaki, F. and Kuwabara, M. 2006. Identification of pH-sensitive regions in the mouse prion by the cystein-scanning spin-labeling ESR technique. Biochem. Biophys. Res. Commun., 350: 549-556.

11) Takahashi, E., Inanami, O., Ohta, T., Matsuda, A. and Kuwabara, M. 2006. Lipid raft disruption prevents apoptosis induced by 2’‐chrolo-2’-deoxyadenosine (Cladribine) in leukemia cell lines. Leuk. Res., 30: 1555-1561.

12) Horikawa, D. D., Sakashita, T., Katagiri, C., Watanabe, M., Kikawada, T., Nakahara , Y., Hamada, N., Wada, S., Funayama, T., Higashi, S., Kobayashi, Y., Okuda, T. amd Kuwabara, M. 2006. Radiation tolerance in the tardigrade Milnesium tardigradum. Int. J. Radiat. Biol., 82: 843-838.

13) Kamibayashi, M., Oowada, S., Kameda, H., Okada, T., Inanami, O., Ohta , S., Ozawa, T., Makino, K. and Kotake, Y. 2006. Synthesis and characterization of a practically better DEPMPO-type spin trap, 5-(2,2-dimethyl-1,3-propoxy cyclophosphoryl)-5-methyl-1-pyrroline N-oxide (CYPMPO). Free Radic. Res., 40: 1166-1172.


Publication other than original articles


1 ) Kuwabara, M., Inanami, O., Yasui, H., Iizuka, D. and Matsuda, A. 2006. Radiation sensitization effects of ethynyl-cytidine. Gann-No-Rinsho, 52: 1-6. (in Japanese).

2 ) Kuwabara, M. 2006. Apoptotic signal transduction induced by singlet oxygen. In Bessatsu-Igakuno-Ayumi.(Oxidative Stress Ver. 2, Ed., T. Yoshikawa) pp.116-119. Ishiyaku Publishers Inc., Tokyo. (in Japanese).



Original Articles


1 ) Kobayashi , S., Sato, R., Inanami, O., Yamamori, T., Yamato, O., Maede, Y., Sato J., Kuwabara, M. and Naito, Y. 2005. Reduction of concanavalin A-induced expression of interferon‐γ by bovine lactoferrin in feline peripheral blood mononuclear cells. Vet. Immunol. Immunophathol., 105: 75-84.

2 ) Hamasu, T., Inanami, O., Asanuma, T. and Kuwabara, M. 2005. Enhanced induction of apoptosis by combined treatment of human carcinoma cells with X rays and death receptor agonists. J. Radiat. Res., 46: 103-110.

3 ) Hamasu, T., Inanami, O., Tsujitani, M., Yokoyama , K., Takahashi, E., Kashiwakura, I. and Kuwabara, M. 2005. Postirradiation hypoxic incubation of Xirradiated MOLT-4 cells reduces apoptotic cell death by changing the intracellular redox state and modulating SAPK/JNK pathways. Apoptosis, 10: 557-568.

4 ) Iizuka, D., Inanami, O., Matsuda, A., Kashiwakura, I., Asanuma, T. and Kuwabara, M. 2005. X irradiation induces the proapoptotic state independent of the loss of clonogenic ability in Chinese hamster V79 cells. Radiat. Res., 164: 36-44.

5 ) Kashiwakura, I., Inanami, O., Abe, Y., Takahashi, T. A. and Kuwabara, M. 2005. Different radiosensitive megakaryocytic progenitor cells exist in steady-state human peripheral blood. Radiat. Res., 164: 10-16.

6) Inanami, O., Hashida, S., Iizuka, D., Horiuchi, M., Hiraoka, W., Shimoyama, Y., Nakamura, H., Inagaki, F. and Kuwabara, M. 2005. Conformational change in fulllength mouse prion : A site-directed spinlabeling study. Biochem. Biophys. Res. Commun., 335: 785-792.


Publication other than original articles


1 ) Niwa, K. and Inanami, O. 2005. Signal transduction pathways and reactive oxygen species in endothelial cells. Radiat. Biol. Res. Commun., 40: 1-13. (in Japanese).



Original Articles


1 ) Nakajima, T., Iwabuchi, S., Miyazaki, H., Okuma, Y., Kuwabara, M., Nomura, Y. and Kawahara, K. 2004. Preconditioning prevents ischemia-induced neuronal death through persistent Akt activation in the penumbra region of the rat brain. J. Vet. Med. Sci., 66:521-527.


2 ) Jalal Hosseinimehr, S., Inanami, O., Hamasu, T., Takahashi, M., Kashiwakura, I., Asanuma, T. and Kuwabara, M. 2004. Activation of C-kit by stem cell factor induces radioresistance to apoptosis through ERK-dependent expression of survivin in HL60 cells. J. Radiat. Res., 45:557-561.


3 ) Inanami, O., Iizuka, D., Iwahara, A., Yamamori, T., Kon, Y., Asanuma, T., Matsuda, A., Kashiwakura, I., Kitazato, K. and Kuwabara, M. 2004. A novel anticancer ribonucleoside, 1-(3-C-ethynyl-beta-D-ribo-pentofuranosyl)cytosine, enhances radiation-induced cell death in tumor cells. Radiat. Res., 162:635-645.


4 ) Cui, Z. G., Kondo, T., Feril, Jr. L. B., Waki, K., Inanami, O. and Kuwabara, M. 2004. Effects of antioxidants on X-ray- or hyperthermia-induced apoptosis in human lymphoma U937 cells.Apoptosis, 9:757-763.


5 ) Asanuma, T., Inanami, O., Tabu, K., Waki, K., Kon, Y. and Kuwabara, M. 2004. Protection against malonate-induced ischemic brain injury in rat by a cell-permeable peptidic c-Jun N-terminal kinase inhibitor, (L)-HIV-TAT48-57-PP-JBD20, observed by the apparent diffusion coefficient mapping magnetic resonance imaging method. Neurosci. Lett., 359:57-60.


6 ) Yamamori, T., Inanami, O., Nagahata, H. and Kuwabara, M. 2004. Phosphoinositide 3-kinase regulates the phosphorylation of NADPH oxidase component p47phox by controlling cPKC/PKCdelta but not Akt. Biochem. Biophys. Res. Comm., 316:720-730.


7 ) Nagahata, H., Higuchi, H., Teraoka, H., Takahashi, K., Kuwabara, M., and Inanami, O. 2004. Decreased apoptosis of beta 2- integrin-deficient bovine neutrophils. Immunol. Cell Biol., 82:32-37.


Publication other than original articles


1 ) Kuwabara, M. 2004. Radiation-induced spatial events and their time scale in biological materials. Jpn. J. Cancer Clin., 50:1051-1054. (in Japanese).


2 ) Inanami, O., Waki, K., Yamamori, T., Asanuma, T., Nakajima T., Nagahata, H. and M. Kuwabara. 2004. Activation mechanism of NADPH oxidase and impaired function of bovine neutrophil after parturition, Vet. Biochem., 41:1-13. (in Japanese).


3 ) Kuwabara, M. 2003. Molecular mechanisms of biodefense against free radicals and reactive oxygens. Akiyama Found. Ann. Rep., 17:23-49. (in Japanese).


4 ) Kuwabara, M., Asanuma, T. and Inanami, O. 2004. Suppression of malonate-induced ischemic brain injury in rat by a cell-permeable peptidic JNK inhibitor: MRI and ADC mapping study. Free Rad. Biol. Med. 36 Suppl:S112-S112.



Original Articles


1 ) Kuwabara, M., Takahashi, K. and Inanami, O. 2003. Induction of Apoptosis through the Activation of SAPK/JNK Followed by the Expression of Death Receptor Fas in X-irradiated Cells. J. Radiat. Res. (Tokyo). 44: 203-209.


2 ) Asanuma, T., Matsumoto, Y., Takiguchi, M., Inanami, O., Nakao, M. Nakaya, K., Ito, A., Hashimto, A. and Kuwabara, M. 2003. Magnetic resonance imaging and immunoblot analysis in rats with experimentally induced cerebral alveolar echinococcosis. Comp. Med. 53: 649-656.


3 ) Niwa, K., Inanami, O., Yamamori, T., Ohta, T., Hamasu, T. and Kuwabara, M. 2003. Redox regulation of PI3K/Akt and p53 in bovine aortic endothelial cells exposed to hydrogen peroxide. Antioxid Redox Signal. 5: 713-722.


4 ) Ogata, M., Inanami, O., Nakajima, M., Nakajima, T., Hiraoka, W. and Kuwabara, M. 2003. Ca(2+)-dependent and caspase-3-independent apoptosis caused by damage in Golgi apparatus due to 2,4,5,7-tetrabromorhodamine 123 bromide-induced photodynamic effects. Photochem. Photobiol. 78: 241-247.


5 ) Waki, K., Inanami, O., Yamamori, T. and Kuwabara, M. 2003. Extracellular signal-regulated kinase 1/2 is involved in the activation of NADPH oxidase induced by FMLP receptor but not by complement receptor 3 in rat neutrophils. Free Radic. Res. 37: 665-671.


6 ) Kashiwakura, I., Inanami, O., Takahashi, K., Takahashi, T.A., Kuwabara, M. and Takagi, Y. 2003. Protective effects of thrombopoietin and stem cell factor on X-irradiated CD34+ megakaryocytic progenitor cells from human placental and umbilical cord blood. Radiat Res. 160: 210-216.


7 ) Sato, R., Inanami, O., Syuto, B., Sato, J., Kuwabara, M. and Naito, Y. 2003. The plasma superoxide scavenging activity in canine cancer and hepatic disease. J. Vet. Med. Sci. 65: 465-469.


8 ) Mizutani, T., Kobayashi, M., Eshita, Y., Inanami, O., Yamamori, T., Goto, A., Ako, Y., Miyoshi, H., Miyamoto, H., Kariwa, H., Kuwabara, M. and Takashima, I. 2003. Characterization of JNK-like protein derived from a mosquito cell line, C6/36. Insect Mol. Biol. 12:61-66.



Original Articles


1 ) Takahashi, K., Inanami, O., Hayashi, M. and Kuwabara, M. 2002. Protein synthesis-dependent apoptotic signaling pathway in X-irradiated human leukemia cell line MOLT-4. Int. J. Radiat. Biol., 78:115-124.


2 ) Kashiwakura, I., Murakami, M., Inanami, O., Hayase, Y., Takahashi, T. A., Kuwabara, M. and Takagi, Y. 2002. Effects of amifostine on the proliferation and diferentiation of megakaryocytic progenitor cells. Eur. J. Pharmacol., 437:19-25.


3 ) Kuwabara, M., Iida, Y., Inanami, O., Sawamura, S., Yokoyama, K. and Tsujitani, M. 2002. Radiation-chemical properties of the hypoxic cell radiosensitizer doranidazole (PR-350). J. Radiat. Res., 43:77-88.


4 ) Inanami, O., Sugihara, K., Okui, T., Hayashi, M., Tsujitani, M. and Kuwabara, M. 2002. Hypoxia and etanidazole alter radiation-induced apoptosis in HL60 cells but not in MOLT-4 cells. Int. J. Radiat. Biol., 78:267-274.


5 ) Yamaguchi, M., Hirayama, F., Murahashi, H., Azuma, H., Sato, N., Miyazaki, H., Fukazawa, K., Sawada, K., Koike, T., Kuwabara, M., Ikeda, H. and Ikebuchi, K. 2002. Ex vivo expansion of human UC blood primitive hematopoietic progenitors and transplantable stem cells using human primary BM stromal cells and human AB serum. Cytotherapy, 4:109-118.


6 ) Yamamori, T., Inanami, O., Sumimoto, H., Akasaki, T., Nagahata, H. and Kuwabara, M. 2002. Relationship between p38 mitogen-activated protein kinase and small GTPase Rac for the activation of NADPH oxidase in bovine neutrophils. Biochem. Biophys. Res. Commun., 293:1571-1578.


7 ) Yamaguchi, M., Hirayama, F., Wakamoto, S., Fujihara, M., Murahashi, H., Sato, N., Ikebuchi, K., Sawada, K., Kuwabara, M. , Azuma, H. and Ikeda, H. 2002. Bone marrow stromal cells prepared using AB serum and bFGF for hematopoietic stem cells expansion. Transfusion, 42:921-927.


8 ) Kashiwakura, I., Inanami, O., Murakami, M., Takahashi, T. A., Kuwabara, M. and Takagi, Y. 2002. Effects of combination of thrombopoietin with cytokines for survival of X-irradiated CD34+ megakaryocytes progenitor cells from normal human peripheral blood. Radiat. Res., 158:202-209.


9 ) Asanuma, T, Ishibashi, H., Konno, A., Kon, S., Inanami, O. and Kuwabara, M. 2002. Assessment of neuroprotective ability of a spin trap, α-phenyl-N-tert-butylnitrone, against malonate-induced ischemic injury of rat brain by apparent water diffusion coefficient mapping. Neurosci. Lett., 329:281-284.


10) Niwa, K., Inanami, O., Yamamori, T., Ohta, T., Hamasu, T., Karino, T. and Kuwabara, M. 2002. Roles of protein kinase Cd in the accumulation of p53 and the induction of apoptosis in H2O2-treated bovine endothelial cells. Free Radic. Res., 36:1147-1153.


11) Nakajima, T., Iwabuchi, S., Miyazaki, H., Yasunobu, O, Inanami, O., Kuwabara, M., Nomura, Y. and Kawahara, K. 2002. Relationship between the activation of CREB and ischemic tolerance in the penumbra region of rat cerebral cortex. Neurosci. Lett., 331:13-16.



Original Articles


1 ) Inanami, O., Yamamori, T., Takahashi, T.A., Nagahata, H. and Kuwabara, M. 2001. ESR detection of intraphagosomal superoxide in polymorphonuclear leukocytes using 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide. Free Radical Res., 34:81-92.


2 ) Yamaguchi, M., Hirayama, F., Kanai, M., Sato, N., Fukazawa, K., Yamashita, K., Sawada, K., Koike, T., Kuwabara, M., Ikeda, H. and Ikebuchi, K. 2001. Serum-free coculture system for ex vivo expansion of human cord blood primitive progenitors and SCID mouse-reconstituting cells using human bone marrow primary stromal cells. Exp. Hematol., 29:174-182.


3 ) Iwasaki, K., Morimatsu, M., Inanami, O., Uchida, E., Syuto, B., Kuwabara, M. and Niiyama, M. 2001. Isolation, characterization, and cDNA cloning of chicken turpentine-induced protein, a new member of the scavenger receptor cystein-rich (SRCR) family of proteins. J. Biol. Chem., 276:9400-9405.


4 ) Asanuma, T., Ohkura, K., Yamamoto, T., Kon, Y., Shimokawa, S. and Kuwabara, M. 2001. Three-dimensional MRI of lung and liver tumors in mice from transversal multislice MR images. Comp. Med., 51:138-144.


5 ) Inanami, O., Yamamori, T., Shionoya, H. and Kuwabara, M. 2001. Antioxidant activity of quinone-derivatives from freeze-dried powder of the ascidians. In: The Biology of Ascidians, pp. 457-462, Rudowicz, Sawada, H., Yokosawa, H. and Lambert, C. C. eds., Springer, Tokyo.


6 ) Abe, H., Ikebuchi, K., Niwa, K., Inanami, O., Kuwabara, M., Fujihara, M., Hirayama, J. and Ikeda, H. 2001. Superoxide generation from human polymorphonuclear leukocytes by liposome-encapsulated hemoglobin. Artif. Cells. Blood Substit. Immobil. Biotechnol., 29:275-283.


7 ) Tsuji, M., Inanami, O. and Kuwabara, M. 2001. Induction of neurite outgrowth in PC12 cells by α-phenyl-N-tert-butylnitrone through activation of protein kinase C and the ras-extracellular-response kinase pathway. J. Biol. Chem., 276:32779-32785.


8 ) Niwa, K., Inanami, O., Ohta, T., Ito, S., Karino, T. and Kuwabara, M. 2001. p38 MAPK and Ca2+ contribute to hydrogen peroxide-induced increase of permeability in vascular endothelial cells but ERK does not. Free Radic. Res., 35:519-527.



Original Articles


1 ) Nomura Y, Inanami O, Takahashi K, Matsuda A and Kuwabara M. 2000. 2-Chloro-2'-deoxyadenosine induces apoptosis through the Fas/Fas ligand pathway in human leukemia cell line MOLT-4. Leukemia, 14:299-306.


2 ) Yamamori T, Inanami O, Nagahata H, Cui Y and Kuwabara M. 2000. Roles of p38 MAPK, PKC and PI3-K in the signaling pathways of NADPH oxidase activation and phagocytosis in bovine polymorphonuclear leukocytes. FEBS Lett., 467:253-238.


3 ) Kashiwakura I, Kuwabara M, Inanami O, Murakami M, Hayase Y, Takahashi TA and Takagi Y. 2000. Radiation sensitivity of megakaryocyte colony-forming cells in human placental and umbilical cord blood. Radiat. Res.,153:144-152.


4 ) Tsuji, M., Inanami, O. and Kuwabara, M. 2000. Neuroprotective effect of α-phenyl-N-tert-butylnitrone in gerbil hippocampus is mediated by the mitogen-activated protein kinase pathway and heat shock proteins. Neurosci. Lett., 282:41-44.


5 ) Iida, Y., Inanami, O., Inoue, H., Ohtsuka, E. and Kuwabara, M. 2000. Unique response of double-stranded oligonucleotides containing a single-8-oxo-7, 8-dihydroguanine to γ-rays in the frozen aqueous state at 77K. Radiat. Res., 154:582-589.


6 ) Kuwabara, M., Takahashi, T. A., Nagahata, H. and Inanami, O. 2000. Spin-trapping detection of superoxides in polymorphonuclear leukocytes stimulated with serum-opsonized zymosan. Jpn. J. Vet. Res., 48:3-13.

7 ) Gao M, Shirato H, Miyasaka K, Kuwabara M, Koyama T. 2000. Induction of growth factors in rat cardiac tissue by X irradiation. Radiat. Res., 153:540-547.


8 ) Kanai, M., Hirayama, F., Yamaguchi, M., Ohkawara, J., Sato, N., Fukazawa, K., Yamashita, K., Kuwabara, M., Ikeda, H. and Ikebuchi, K. 2000. Stromal cell-dependent ex vivo expansion of human cord blood progenitors and augmentation of transplantable stem cell activity. Bone Marrow Transplant., 26:837-844.


9 )Cui, Y.-D., Inanami, O., Yamamori, T., Niwa, K., Nagahata, H. and Kuwabara, M. 2000. FMLP-induced formation of F-actin in HL60 cells is dependent on PI3-K but not on intracellular Ca2+, PKC, ERK or p38 MAPK. Inflamm. Res., 49:684-691.



Original Articles


1 ) Takahashi, K., Inanami, O. and Kuwabara, M. 1999. Effects of intracellular calcium chelator BAPTA-AM on radiation-induced apoptosis regulated by activation of SAPK/JNK and caspase- 3 in MOLT- 4 cells. Int. J Radiat. Biol ., 75 : 1099-1105.


2 ) Inanami, O., Yoshito, A., Takahashi, K., Hiraoka W. and Kuwabara, M. 1999. Effects of BAPTA-AM and forskolin on apoptosis and cylochrome c release in photosensitized Chinese hamster V79 cells. Photochem. Photobiol ., 70 : 650-655.


3 ) Matsuki, N.,Takanohashi, A., Boffl, F. M., Inanami, O., Kuwabara, M. and Ono, K. 1999. Hydroxyl radical generation and lipid peroxidation in C 2 C12 myotube treated with iodoacetate and cyanide. Free Radic. Res.,31 : I -8.


4 ) Inanami, O., Shiga, A., Okada, K., Sato, R., Miyake, Y. and Kuwabara, M. 1999. Lipid peroxides and antioxidants in serum of neonatal calves. Am. J Vet. Res ., 60 : 452-457.


5 ) Inanami , O., Takahashi, K, and Kuwabara, M. 1999. Attenuation of caspase- 3 -dependent apoptosis by Trolox post-treatment of X-irradiated MOLT- 4 cells. Int. J Radiat. Biol ., 75 : 155-163.


6 ) Okada, F., Nakai, K., Kobayashi, T., Shibata, T., Tagami, S., Kawakami, Y., Kitazawa, T., Kominami, R., Yoshimura, S., Suzuki, K., Taniguchi, N., Inanami, O., Kuwabara, M., Klshida, H., Nakae, D., Konishi, Y., Moriuchi, T. and Hosokawa, " M. 1999. Inflammatory cell-mediated tumour progression and minisatellite mutation correlate with the decrease of anti-oxidative enzymes in murine fibrosarcoma cells. Br. J Cancer, 79 : 377-385.


7 ) Inanami, O., Ohta, T., Ito, S. and Kuwabara, M. 1999. Elevation of intracellular calcium ion is essential for the H202-induced activation of SAPK / JNK but not for that of p38 and ERK in Chi-nese hamster V79 cells. Antiox. Redox Signal ., I : 501-508.


8 ) Inanami, O., Takahashi, K., Yoshito, A. and Kuwabara, M. 1999. Hydrogen peroxide-induced activation of SAPK/JNK regulated by phosphatidylinositol 3 -kinase in Chinese hamster V79 cells. Antiox. Redox Signal ., I : 113-121.


9 ) Asanuma T, Hirano Y, Yamamoto K, Kon Y, Shimokawa S, Kuwabara M. 1999. MR imaging of hepatic injury in the LEC rat under a high magnetic field (7.05T) . J Vet. Med . Sci.,61 : 239-244.


10) Inanami, O., Takahashi, K., Yoshito, A. and Kuwabara, M. 1999. Intracellular signal transduction pathway induced by oxidative stress in Chinese hamster V79 lung fibroblast. Mag. Res. Med ., 10 : 33-37. (in Japanese with English summary)


11) Yoshito, A., Hiraoka, W., Inanami, O. and Kuwabara, M. 1999. Apoptosis induced in Chinese hamster V79 cells by the photodynamic treatment with pheophorbide a is mediated by MAP kinase cascade. Mag. Res. Med .,


10 : 38-42. (in Japanese with English summary)


12) Takahashi, K., Inanami, O., Iida, Y., Tsuji, M. and Kuwabara, M. 1999. The effects of intracellular Ca2+ in radiation-induced apoptosis of MOLT- 4 cells. Mag. Res. Med ., 10 : 43-47. (in Japanese with English summary)


13) Watanabe, D., Inanami, O., Sawamura, S., Ozawa, T. and Kuwabara, M. 1999. ESR and spin-trapping study of radiation-induced free radicals in polyA/polyU. Mag. Res. Med ., 10 : 69-73. (in Japanese with English summary)



Original Articles


1 ) Asanuma, T., Shimokawa, S., Inanami, O., Kon, Y. and Kuwabara. M. 1998. Visualization of the topographical structure of the anesthetized mouse brain by MR microimaging. J . Vet. Med. Sci., 60:1311-1334.


2 ) Ogawa, A., Shuto, S., Inanami, O., Kuwabara, M., Tanaka, M., Sasaki, T. and Matsuda, A. 1998. Nucleosides and nucleotides. 176. 2'-Deoxy-2'-hydroxylaminocytidine: a new antitumor nucleoside that inhibits DNA synthesis although it has a ribonucleoside structure. Bioorg. Med. Chem. Lett., 8:1913-1918.


3 ) Inanami, O., Watanabe, Y., Syuto, B., Nakano, M., Tsuji, M. and Kuwabara, M. 1998. Oral administration of (-)catechin protects against ischemia-reperfusion-induced neuronal death in the gerbil. Free Radical Res. 29:359-365.


4 ) Inanami, O., Johnson, J.L., McAdara, J.K., Benna, J.E., Faust, L.R., Newburger, P.E. and Babior, B.M. 1998. Activation of the leukocyte NADPH oxidase by phorbol ester requires the phosphorylation of p47PHOX on serine 303 or 304. J. Biol. Chem., 273:9539-9543.


5 ) Johnson, J.L., Park, J.W., Benna, J.E., Faust, L.P., Inanami, O. and Babior, B.M. 1998. Activation of p47(PHOX), a cytosolic subunit of the leukocyte NADPH oxidase. Phosphorylation of ser-359 or ser-370 precedes phosphorylation at other sites and is required for activity. J. Biol. Chem., 273:35147-35152.


6 ) Nagahata. H., Matsuki, S., Higuchi, H., Inanami, O., Kuwabara, M. and Kobayashi, K. Bone marrow transplantation in a Holstein heifer with bovine leucocyte adhesion deficiency. Vet. J., 156:15-21.


7 ) Inanami, O., Johnson, J.L. and Babior, B.M. 1998. The leukocyte NADPH oxidase subunit p47PHOX: the role of the cysteine residues.. Arch. Biochem. Biophys. 350:36-40.


8 ) Cho, K.O., Park, N.Y., Endoh, D., Ohashi, K., Sugimoto, C., Itakura, C. and Onuma, M. 1998. Cytology of feather pulp lesions from Marek's disease (MD) virus-infected chickens and its application for diagnosis and prediction of MD. J . Vet. Med. Sci., 60:843-847.


9 ) Ui, M., Endoh, D., Cho, K.O., Kon, Y., Iwata, A., Maki, Y., Sato, F. and Kuwabara, M. 1998. Transcriptional analysis of Marek's disease virus (MDV) genes in MDV-transformed lymphoblastoid cell lines without MDV-activated cells. J . Vet. Med. Sci., 60:823-829.


10) Kon, Y., Endoh, D., Yamashita, T. and Watanabe, T. 1998. Expression of renin in the rat liver. Anat. Histol. Embryol. 27:111-118.


11) Endoh, D., Ito, M., Cho, K.O., Kon, Y., Morimura, T., Hayashi, M. and Kuwabara, M. 1998. Retroviral sequence located in border region of short unique region and short terminal repeat of Md5 strain of Marek's disease virus type 1. J . Vet. Med. Sci., 60:227-235.


12) Kashiwakura, I., Kuwabara, M., Murakami, M., Hayase, Y. and Takagi, Y. 1998. Effect of carboxy-PTIO, a nitric oxide scavenger, on the proliferation of murine hematopoietic progenitor cells in vitro. Res. Commun. Mol. Pathol. Pharmacol., 99:329-337.


13) Nakai, K., Usuba, A., Ohta, T., Kuwabara, M., Nakazato, Y., Motoki, R. and Takahashi, T. 1998. Coronary vascular bed perfusion with a polyethylene glycol modified hemoglobin-encapsulated liposome, neo red cell, in rats. Artificial Organs, 22:320-325.


14) Kuwabara, M., Ohshima, H., Iida, Y. and Inanami, O. 1998. Roles of water molecules in the hydration layer of DNA in the formation of radiation-induced DNA damage. Mag. Res. Med., 9:102-105 (in Japanese with English summary)


15) Inanami, O., Asanuma, T. Kotake Y. and Kuwabara, M. 1998. Effects of aging on nitric oxide production in LPS-treated Wistar rats. Mag. Res. Med., 9:13-16 (in Japanese with English summary)


Publication other than original articles


1 ) Kuwabara, M., Inanami, O., Takahashi, K. and Hiraoka, W. 1998. Radiation- and reactive oxygen-induced cell death and signal transduction in cultured mammalian cells. In: Biological Oxidants & Antioxidants: Molecular Mechanisms & Health Effects, pp. 14-21, Packer, L. & Ong, H.S. Eds., AOCS Press, Illinois.


2 ) Kuwabara, M. and Inanami, O. 1998. Biodefence against active oxygens and free radicals induced by oxidative stress. In: Biological Mechanisms against Environmental Stress, pp. 23-32, Ozawa, T., Tatsumi, K. and Hori, T. eds., Kodansha and Springer, Tokyo.



Original Articles


1 ) Kashiwakura, I., Kuwabara, M., Murakami, M., Hayase, Y. and Takagi, Y. 1997. Effects of a-phenyl-N-tert-butylnitrone, a spin trap reagent, on the proliferation of murine hematopoietic progenitor cells in vitro. Res. Comms. Mol. Path. Pharm., 98: 67-76.


2 ) Asanuma, T., Hirano, Y., Ohkura, K., Kon, Y., Shimokawa, S., Kasai, N. and Kuwabara, M. 1997. Magnetic resonance imaging of hepatocellular carcinoma in Long-Evans Cinnamon rat under a magnetic field of 7.05 T. Jpn. J. Vet. Res., 45: 147-151.


3 ) Kuwabara, M., Ohshima, H., Iida, Y. and Inanami, O. 1997. The increased formation of alkali-labile sites by a spin-trapping agent a-phenyl-N-tert- butylnitrone in g-irradiated frozen aqueous solution of DNA. J. Radiat. Res., 38: 153-155.


4 ) Yamashita, T., Yamazaki, H., Kon, Y., Watanabe, T., Arikawa, J., Miyoshi, I., Kasai, N. and Kuwabara, M. 1997. Progressive effect of a-phenyl-N-tert-butylnitrone (PBN) on rat embryo development in vitro. Free Rad. Biol. Med., 23: 1073-1077.


5 ) Abe, H., Ikebuchi, K., Wagner, S. J., Kuwabara, M., Kamo, N. and Sekiguchi, S. 1997. Involvement of both type I and type II mechanisms in M13 virus inactivation by methylene blue photosensitization. Photochem. Photobiol., 66: 204-208.


6 ) Hiraoka, W., Fuma, K. and Kuwabara, M. 1997. Concentration-dependent modes of cell death in Chinese hamster V79 cells after treatments with H2O2. J. Radiat. Res., 38: 95-102.


7 ) Abe, H., Wagner, S. J., Kuwabara, M., Kamo, N., Ikebuchi, K. and Sekiguchi, S. 1997. Factors affecting M13 bacteriophage inactivation by methylene blue photosensitization. Photochem. Photobiol., 65: 873-876.


8 ) Ohshima, H., Ono, A., Matsuda, A., Sawamura, S. and Kuwabara, M. 1997. Reactions between hydroxyl-radical-induced 7,8-dihydro-8-oxo-2'-deoxyguanosine precursor and the spin trap a-phenyl-N-tert-butylnitrone. J. Radiat. Res., 38: 15-25.


9 ) Kuwabara, M., Goji, N., Inanami, O., Higuchi, H. and Nagahata, H. 1997. Analysis of signal transduction mechanisms leading to the reactive oxygen generation by employing neutrophils from a Holstein calf with bovine leukocyte adhesion deficiency (BLAD). Mag. Res. Med., 8: 109-113 (in Japanese with English summary).


10) Cho, K.-O., Endoh, D., Kimura, T., Ochiai, K. and Itakura, C. 1997. Significance of Marek's disease virus serotype 1-specific phosphorylated proteins in the Marek's disease skin lesions. Avian Pathol., 26: 707-720.


11) Hayashi, M., Uehara, K., Kirisawa, R., Endoh, D., Arai, S. and Okui, T. 1997. Abnormal G1 arrest in the cell lines from LEC strain rats after X-irradiation. J. Vet. Med. Sci., 59: 769-773.


Publications other than original articles


1 ) Kuwabara, M., Goji, N., Inanami, O., Higuchi, H. and Nagahata, H. 1997. An ESR study of superoxide generation in stimulated neutrophils from a calf with bovine leukocyte adhesion deficiency (BLAD). In: Modern Applications of EPR/ESR From Biophysics to Material Science, pp. 46-53, Rudowicz, C. Z., Yu, K. N. and Hiraoka, H. eds., Springer, Singapore.


2 ) Asanuma, T. and Kuwabara, M. 1997. Magnetic resonance imaging of small laboratory animals under a magnetic field of 7.05 T. Radiol. Sci., 40: 221-227 (in Japanese).


3 ) Kuwabara, M. 1997. Free radicals in vivo. Radiat. Biol. Res. Comms. 32:133-141 (in Japanese).


4 ) Kuwabara, M. 1997. Reactions induced in DNA by oxyl radicals: Detection and identification of precursor radicals of DNA damage by the spin-trapping method. Chem. Biol., 35: 91-98 (in Japanese).



Original articles


1 ) Kuwabara, M., Inukai, N., Inanami, O., Miyake, Y., Tstunoda, N., Maki, Y. and Sato, F. 1996. Lipid peroxide levels and superoxide-scavenging abilities of sera obtained from hotbred (Thoroughbred) horses. J. Vet. Med. Sci., 58: 97-101.


2 ) Nagahata, H., Higuchi, H., Goji, N., Noda, H. and Kuwabara, M. 1996. Functional characteristics of enhanced Fc receptor expression of ß2 integrin-deficient bovine mononuclear phagocyte. Microbiol. Immunol., 40: 389-395.


3 ) Nagahata, H., Higuchi, H., Noda, H., Tamoto, K. and Kuwabara, M. 1996. Adhesiveness of extracellular matrices and Iysosomal enzyme release from normal and ß2 integrin-deficients bovine neutrophils. Microbiol. Immunol., 40: 783-786.


4 ) Yamashita, T., Ohshima, H., Asanuma, T., Inukai, N., Miyoshi, I., Kasai, N., Kon, Y., Watanabe, T., Sato, F. and Kuwabara, M. 1996. The effects. of a-phenyl-tert-butyl nitrone (PBN) on copper-induced rat fulminant hepatitis with jaundice. Free Radic. Biol. Med., 21: 755-761.


5 ) Ohshima, H., lida, Y., Matsuda, A. and Kuwabara, N. 1996. Damage induced by hydroxyl radicals generated in the hydration layer of gamma-irradiated frozen aqueous solution of DNA. J. Radiat. Res., 37: 199-207.


6 ) Suzuki, T., Goryo, M., Inanami, O., Uetsuki, J., Saito, S., Kaneda, K., Oshima, T., Shimizu, H., Okabe, S., Tanaka, T., Kamata, R., Syuto, F., Sato, I., Tachikawa, E., Sakagutchi, M., Kobayashi, H. and Okada, K. 1996. Inhibition of collagen-induced platelet aggregation in Japanese black cattle with inherited platelet disorder, Chediak-Higashi syndrome. J. Vet. Med. Sci., 58: 647-654.


7 ) Sato, R. Inanami, O. Tanaka, Y. Takase, M. and Naito Y. 1996. Oral administration of bovine lactoferrin for treatment of intractable stomatitis in feline immunodeficiency virus (FIV)-positive and FIV-negative cats. Am. J. Vet. Res. 57: 1443-1446.


8 ) Iwasaki, T., Hashimoto, N., Endoh, D., Imanishi, T., Itakura, C. and Sato, F. 1996. Life span and tumours in the first-generation offspring of the É¡-irradiated male mouse. Int. J. Radiat. Biol. 69: 487-492.


9 ) Okui, T., Endoh, D., Arai, S. and Hayashi, M. 1996. A high frequency of induction of chromosome aberration in the fibroblast of LEC strain rats by X-irradiation. J. Vet. Med Sci., 58: 795-797.


10) Kon, Y., Endoh, D., Fukamizu, A., Murakami, K., Yamashita, T. and Watanabe, T. 1996. Detection of coagulation gland renin by hydridohistochemistry. Anal. Histol. Embryol., 25. 289-294.


11) Endo, D., Kon, Y., Hayashi, M., Morimura, T., Cho, K.-O., Iwasaki, T. and Sato, F. 1996. Detection of transcripts of Marek's desease virus serotype 1 ICP4 homologue (MDV1 ICP4) by in situ hybridization. J. Vet. Med. Sci. , 58: 969-975.


12) Kojima, M., Inanami, O. Suzuki, K., Hiraoka, W., Syuto, B. and Kuwabara, M. 1996. The small molecular weight GTP-binding proteins (Rho) regulate the superoxide production from polymorphonuclear leukocytes. Mag Res. Med. 7: 113-116 (in Japanese).


13) Inanami, O., Sasaki, M., Kojima, M., Okada, K., Syuto, B. and Kuwabara, M. 1996.: The superoxide production from opsonized-zymosan- and PMA-stimulated polymorphonuclear leukocyte in neonatal calf. Mag. Res. Med., 7: 117-120 (in Japanese).


14) Inanami, O. and Kuwabara, M. 1996. Localization of O2- and OH radicals in polymorphonuclear leukocytes stimulated by opsonized zymosan. Proc. Jpn. Soc. Animal. Biochem. 32: 43-50 (in Japanese).


Publications other than original articles


1 ) Kuwabara, M. 1996. Radiation-induced DNA damage. In: Modeling and Biological Effects and Risks of Radiation Exposure, pp. 14-24, Inaba, J. and Kobayashi, S. eds., NIRS, Chiba (in Japanese).


2 ) Hiraoka, W., Sato, F. and Kuwabara, M., 1996. Apoptosis and necrosis induced by reactive oxygen species. In: Special Issue of Jap. J. Vet. Pathol., pp. 14-17 (in Japanese).