Original articles


1 ) Uemura M, Ochiai K, Morimatsu M, Michishita M, Onozawa E, Azakami D, Uno Y, Yoshikawa Y, Sasaki T, Watanabe M, Omi T. The canine RAD51 mutation leads to the attenuation of interaction with PALB2. Vet Comp Oncol 18(2), 247-255, 2020. (doi: 10.1111/vco.12542)



Original articles


1 ) Boonyarattanasoonthorn T, Elewa YHA, Tag-El-Din-Hassan HT, Morimatsu M, Agui T. Profiling of cellular immune responses to Mycoplasma pulmonis infection in C57BL/6 and DBA/2 mice. Infect Genet Evol 73, 55-65, 2019. (doi: 10.1016/j.meegid.2019.04.019)

2 ) Wang J, Dang R, Miyasaka Y, Hattori K, Torigoe D, Okamura T, Tag-Ei-Din-Hassan HT, Morimatsu M, Mashimo T, Agui T. Null mutation of the endothelin receptor type B gene causes embryonic death in the GK rat. PLoS One 14, e0217132, 2019. (doi: 10.1371/journal.pone.0217132)



Original articles


1 ) Abbas RMF, Tag-El-Din-Hassan HT, Boonyarattanasoonthorn T, Aoshima K, Morimatsu M, Agui T. Immune cellular responses to Sendai virus infection in D2.B6-Sen1Sen2Sen3 congenic mice, of which three quantitative trait loci responsible for the resistance to infection were introgressed from C57BL/6 mouse into DBA/2 mouse. Jpn J Vet Res 66(2), 93-103, 2018. (doi: 10.14943/jjvr.66.2.93)


2 ) Abbas RMF, Torigoe D, Kameda Y, Tag-El-Din-Hassan HT, Sasaki N, Morimatsu M, Agui T. Verification of genetic loci responsible for the resistance/susceptibility to the Sendai virus infection using congenic mice. Infect Genet Evol 57, 75-81, 2018. (doi: 10.1016/j.meegid.2017.11.008)


3 ) Islam MA, Torigoe D, Kameda Y, Irie T, Kouguchi H, Nakao R, Masum MA, Ichii O, Kon Y, Tag-El-Din-Hassan HT, Morimatsu M, Yagi K, Agui T. Analysis for genetic loci controlling protoscolex development in the Echinococcus multilocularis infection using congenic mice . Infect Genet Evol 65, 65-71, 2018. (doi: 10.1016/j.meegid.2018.07.017)


4 ) Kawakami S, Ochiai K, Azakami D, Kato Y, Michishita M, Morimatsu M, Ishiguro-Oonuma T, Onozawa E, Watanabe M, Omi T. R132 mutations in canine isocitrate dehydrogenase 1 (IDH1) lead to functional changes. Vet Res Commun 42(1), 49-56, 2018. (doi: 10.1007/s11259-017-9707-8)


5 ) Ochiai K, Azakami D, Morimatsu M, Hirama H, Kawakami S, Nakagawa T, Michishita M, Egusa AS, Sasaki T, Watanabe M, Omi T. Endogenous Leu332Gln mutation in p53 disrupts the tetramerization ability in a canine mammary gland tumor cell line . Oncol Rep 40(1), 488-494, 2018. (doi: 10.3892/or.2018.6409)


6 ) Tag-El-Din-Hassan HT, Morimatsu M, Agui T. Functional analysis of duck, goose, and ostrich 2’-5’ oligoadenylate synthetase. Infect Genet Evol 62, 220-232, 2018. (doi: 10.1016/j.meegid.2018.04.036)



Original articles


1 ) Fujimoto Y, Tomioka Y, Ozaki K, Takeda K, Suyama H, Yamamoto S, Takakuwa H, Morimatsu M, Uede T, Ono E. Comparison of the antiviral potential among soluble forms of herpes simplex virus type-2 glycoprotein D receptors, herpes virus entry mediator A, nectin-1 and nectin-2, in transgenic mice. J. Gen. Virol 98, 1815-1822, 2017. (DOI: 10.1099/jgv.0.000804)


2 ) Kato Y, Ochiai K, Kawakami S, Nakao N, Azakami D, Bonkobara M, Michishita M, Morimatsu M, Watanabe M, Omi T. Canine REIC/Dkk-3 interacts with SGTA and restores androgen receptor signalling in androgen-independent prostate cancer cell lines. BMC Vet. Res 13, 170, 2017. (DOI: 10.1186/s12917-017-1094-4)


3 ) Okamatsu-Ogura Y, Fukano K, Tsubota A, Nio-Kobayashi J, Nakamura K, Morimatsu M, Sakaue H, Saito M, Kimura K. Cell-cycle arrest in mature adipocytes impairs BAT development but not WAT browning, and reduces adaptive thermogenesis in mice. Sci. Rep  7, 6648, 2017. (DOI: 10.1038/s41598-017-07206-8)


4 ) Tag-El-Din-Hassan HT, Sasaki N, Torigoe D, Morimatsu M, Agui T. Analysis of the Relationship Between Enzymatic and Antiviral Activities of the Chicken Oligoadenylate Synthetase-Like. J. Interferon Cytokine Res 37, 71-80, 2017. (DOI: 10.1089/jir.2016.0012)



Original articles


1 ) Ochiai, K., Morimatsu, M., Kato, Y., Ishiguro-Oonuma, T., Udagawa, C., Rungsuriyawiboon, O., Azakami, D., Michishita, M., Ariyoshi, Y., Ueki, H., Nasu, Y., Kumon, H., Watanabe, M. and Omi, T. 2016. Tumor suppressor REIC/DKK-3 and co-chaperone SGTA: Their interaction and roles in the androgen sensitivity. Oncotarget, 7: 3283-3296. (doi: 10.18632/oncotarget.6488)


2 ) Huang J, Dang R, Torigoe D, Li A, Lei C, Sasaki N, Wang J, Agui T. 2016. Genetic variation in the GDNF promoter affects its expression and modifies the severity of Hirschsprung's disease (HSCR) in rats carrying Ednrbsl mutations. Gene, 57: 144-148. (doi: 10.1016/j.gene.2015.08.051)


3 ) Tomioka, Y., Fujimoto, Y., Nakai, K., Ozaki, K., Yamamoto, S., Suyama, H., Morimatsu, M., Ito, T. and Ono, E. 2016. A soluble form of human nectin-2 impairs exocrine secretion of pancreas and formation of zymogen granules in transgenic mice. Biochem. Biophys. Rep., 5: 196–202. (doi: 10.1016/j.bbrep.2015.12.006)


4 ) Huang J, Dang R, Torigoe D, Li A, Lei C, Sasaki N, Wang J, Agui T. QTL analysis of modifiers for pigmentary disorder in rats carrying Ednrbsl mutations.Sci. Rep.6, 19697, 2016.(DOI: 10.1038/srep19697)


5) Sasaki H, Kimura J, Nagasaki K, Marusugi K, Agui T, Sasaki N. Mouse chromosome 2 harbors genetic determinants of resistance to podocyte injury and renal tubulointerstitial fibrosis.BMC Genet.17, 69-79, 2016.(DOI: 10.1186/s12863-016-0378-1)


6) Ono M, Sasaki H, Nagasaki K, Torigoe D, Ichii O, Sasaki N, Agui T. Does the routine handling affect the phenotype of disease model mice?Jpn. J. Vet. Res. 64, 265-271, 2016. (DOI:10.14943/jjvr.64.4.265)


7) Elkhateeb E, Tag-El-Din-Hassan HT, Sasaki N, Torigoe D, Morimatsu M, Agui T. The role of mouse 2',5'-oligoadenylate synthetase 1 paralogs. Infect. Genet. Evol 45, 393-401, 2016. (DOI: 10.1016/j.meegid.2016.09.018)


8 ) Fujimoto Y, Tomioka Y, Takakuwa H, Uechi G, Yabuta T, Ozaki K, Suyama H, Yamamoto S, Morimatsu M, Mai le Q, Yamashiro T, Ito T, Otsuki K, Ono E. Cross-protective potential of anti-nucleoprotein human monoclonal antibodies against lethal influenza A virus infection. J. Gen. Virol 97, 2104-2116, 2016. (DOI: 10.1099/jgv.0.000518)


9 ) Saito M, Yamamoto S, Ozaki K, Tomioka Y, Suyama H, Morimatsu M, Nishijima KI, Yoshida SI, Ono E. A soluble form of Siglec-9 provides a resistance against Group B Streptococcus (GBS) infection in transgenic mice. Microb. Pathog 99, 106-110, 2016. (DOI: 10.1016/j.micpath.2016.08.014)



Original articles


1 ) Abou-Elnaga, A. F., Torigoe, D., Fouda, M. M., Darwish, R. A., Abou-Ismail, U. A., Morimatsu, M. and Agui, T. 2015. Identification of multiple genetic loci in the mouse controlling immobility time in the tail suspension and forced swimming tests. Jpn. J. Vet. Res., 63: 53-62. (doi: 10.14943/jjvr.63.2.53)


2 ) Huang, J., Dang, R., Torigoe, D., Lei, C., Lan, X., Chen, H., Sasaki, N., Wang, J. and Agui, T. 2015. Identification of genetic loci affecting the severity of symptoms of Hirschsprung disease in rats carrying Ednrbsl mutations by quantitative trait locus analysis. PLoS One, 10: e0122068. (doi: 10.1371/journal.pone.0122068)


3 ) Ishiguro-Oonuma, T., Ochiai, K., Hashizume, K. and Morimatsu, M. 2015. The role of IFN-gamma in regulating Nfkbiz expression in epidermal keratinocytes. Biomed. Res., 36: 103-107. (doi: 10.2220/biomedres.36.103)


4 ) Ochiai, K., Ishiguro-Oonuma, T., Yoshikawa, Y., Udagawa, C., Kato, Y., Watanabe, M., Bonkobara, M., Morimatsu, M. and Omi, T. 2015. Polymorphisms of canine BRCA2 BRC repeats affecting interaction with RAD51. Biomed. Res., 36: 155-158. (doi: 10.2220/biomedres.36.155)


5 ) Yoshikawa, Y., Morimatsu, M., Ochiai, K., Ishiguro-Oonuma, T., Wada, S., Orino, K. and Watanabe, K. 2015. Reduced canine BRCA2 expression levels in mammary gland tumors. BMC Vet. Res., 11: 159. (doi: 10.1186/s12917-015-0483-9)


6 ) Kato, Y., Michishita, M., Azakami, D., Nakahira, R., Morimatsu, M., Ishiguro-Oonuma, T., Yoshikawa, Y., Kobayashi, M., Bonkobara, M., Kobayashi, M., Takahashi, K., Watanabe, M. and Omi, T. 2015. Molecular cloning of canine co-chaperone SGTA and investigation of its ability to suppress androgen receptor signalling in androgen-independent prostate cancer. Vet. J., 206: 143-148. (doi: 10.1016/j.tvjl.2015.08.002)


7 ) Ishiguro-Oonuma, T., Ochiai, K., Hashizume, K., Iwanaga, T. and Morimatsu M. 2015. Nfkbiz regulates the proliferation and differentiation of keratinocytes. Jpn. J. Vet. Res., 63: 107-114. (doi: 10.14943/jjvr.63.3.107)


8 ) Sasaki, H., Marusugi, K., Kimura, J., Kitamura, H., Nagasaki, K., Torigoe, D., Agui, T. and Sasaki, N. 2015. Genetic background-dependent diversity in renal failure caused by the tensin2 gene deficiency in the mouse. Biomed. Res., 36: 323-330. (doi: 10.2220/biomedres.36.323)



Original articles


1 ) Asano, A., Torigoe, D., Sasaki, N. and Agui, T. 2014. Development of a sensitive ELISA using a recombinant P46-like lipoprotein for diagnosis of Mycoplasma pulmonis infection in rodents. J. Vet. Med. Sci., 76: 151-157. (doi: 10.1292/jvms.13-0308)


2 ) Sasaki, H., Sasaki, N., Nishino, T., Nagasaki, K., Kitamura, H., Torigoe, D. and Agui, T. 2014. Quantitative trait loci for resistance to the congenital nephropathy in tensin 2-deficient mice. PLoS One, 9: e99602. (doi: 10.1371/journal.pone.0099602)


3 ) Tomioka, Y., Morimatsu, M., Nishijima, K., Usui, T., Yamamoto, S., Suyama, H., Ozaki, K., Ito, T. and Ono, E. 2014. A soluble form of Siglec-9 provides an antitumor benefit against mammary tumor cells expressing MUC1 in transgenic mice. Biochem. Biophys. Res. Commun., 450: 532-537. (doi: 10.1016/j.bbrc.2014.06.009)


4 ) Tanaka, T., Kasai, H., Yamashita, A., Okuyama-Dobashi, K., Yasumoto, J., Maekawa, S., Enomoto, N., Okamoto, T., Matsuura, Y., Morimatsu, M., Manabe, N., Ochiai, K., Yamashita, K. and Moriishi, K. 2014. Hallmarks of hepatitis C virus in equine hepacivirus. J. Virol., 88: 13352-13366. (doi: 10.1128/JVI.02280-14)



Original articles


1 ) Yoshii, K., Moritoh, K., Nagata, N., Yokozawa, K., Sasaki, N., Kariwa, H., Agui, T. and Takashima, I. 2013. Susceptibility to flavivirusspecific antiviral response of Oas1b affects the neurovirulence of the Far-Eastern subtype of tick-borne encephalitis virus. Arch. Virol., 158: 1039-1046.


2 ) Sasaki, N., Yamauchi, H., Nishino, T. and Agui, T. 2013. The telocentric tandem repeat at p-arm is not conserved in the Mus musculus subspecies. Gene, 513: 214-218.


3 ) Asano, A., Torigoe, D., Sasaki, N. and Agui, T. 2013. Epitope mapping of the nucleocapsid protein of Sendai virus and application of antigenic epitopes for the ELISA-based diagnosis of Sendai virus infection. J. Vet. Med. Sci., 75: 909-916.



Original articles


1 ) Dang, R., Sasaki, N., Torigoe, D. and Agui, T. 2012. Anatomic modifications in the enteric nervous system of JF1 mice with the classic piebald mutation. J. Vet. Med. Sci.74: 391-394.

2 ) Nishino, T., Sasaki, N., Nagasaki, K., Ichii, O., Kon, Y. and Agui, T. 2012. The 129 genetic background affects susceptibility to glomerulosclerosis in tensin2-deficient mice. Biomed. Res.33: 53-56.

3 ) Hosoda, Y., Sasaki, N., Kameda, Y., Torigoe, D. and Agui, T. 2012. Identifying quantitative trait loci affecting resistance to congenital hypothyroidism in 129+Ter/SvJcl strain mice. PLoS ONE 7: e31035.

4 ) Nishino, T., Sasaki, N., Chihara, M., Nagasaki, K., Torigoe, D., Kon, Y. and Agui, T. 2012. Distinct distribution of the tensin family in the mouse kidney and small intestine. Exp. Anim.61: 525-532.

5 ) Dang, R., Sasaki, N., Nishino, T., Nakanishi, M., Torigoe, D. and Agui, T. 2012. Lymphopenia in Ednrb-deficient rat was strongly modified by genetic background. Biomed. Res.33: 249-253.

6 ) Tag-El-Din-Hassan, H.T., Sasaki, N., Moritoh, K., Torigoe, D., Maeda, A. and Agui, T. 2012. The chicken 2’-5’ oligoadenylate synthetase A inhibits the replication of West Nile Virus. Jpn. J. Vet. Res.60: 95-103. 



Original articles


1 ) Moritoh, K., Maeda, A., Nishino, T., Sasaki, N. and Agui, T. 2011. Development and application of West Nile virus subgenomic replicon RNA expressing secreted alkaline phosphataseJ. Vet. Med. Sci., 73: 683-686. 

2 ) Torigoe, D., Ichii, O., Dang, R., Ohnaka, T., Okano, S., Sasaki, N., Kon, Y. and Agui, T. 2011. High-resolution linkage mapping of the rat hooded locus. J. Vet. Med. Sci., 73: 707-710. 

3 ) Simon, A.Y., Sasaki, N., Ichii, O., Kajino, K., Kon, Y. and Agui, T. 2011. Distinctive and critical roles for cellular immunity and immune-inflammatory response in the immunopathology of Sendai virus infection in mice. Microbes Infect.13: 783-797. 

4 ) Nakao, R., Kameda, Y., Kouguchi, H., Simon, A.Y., Matsumoto, J., Dang, Z., Torigoe, D., Sasaki, N., Oku. Y., Sugimoto, C., Agui, T. and Yagi, K. 2011. Identification of genetic loci affecting the establishment and development of Echinococcus multilocularis larvae in mice by quantitative trait loci analysis. Int. J. Parasitol.41: 1121-1128. 

5 ) Asano, A., Torigoe, D., Sasaki, N. and Agui, T. 2011. Identification of antigenic peptides derived from B-cell epitopes of nucleocapsid protein of mouse hepatitis virus for serological diagnosis. J. Virol. Meth.177: 107-111. 

6 ) Dang, R., Torigoe, D., Suzuki, S., Kikkawa, Y., Moritoh, K., Sasaki, N. and Agui, T. 2011. Genetic background strongly modifies the severity of symptoms of Hirschsprung disease, but not hearing loss in rats carrying Ednrbsl mutations. PLoS One 6: e24086. 

7 ) Dang, R., Torigoe, D., Sasaki, N. and Agui, T. 2011. QTL analysis identifies a modifier locus of aganglionosis in the rat model of Hirschsprung disease carrying Ednrbslmutations. PLoS One 6: e27902. 

8 ) Ichii, O., Yabuki, A., Sasaki, N., Otsuka, S., Ohta, H., Yamasaki, M., Takiguchi, M., Namiki, Y., Hashimoto, Y., Endoh, D. and Kon, Y. 2011. Pathological correlations between podocyte injuries and renal functions in canine and feline chronic kidney diseases. Histol. Histopathol.26: 1243-1255. 



Original articles


1 ) Ichii, O., Kon, Y., Otsuka, S., Sasaki, N., Hashimoto, Y., Ohta, H., Takiguchi, M. and Endoh, D. 2010. Local overexpression of interleukin 1 family, member 6 related to the development of tubulointerstitial lesionsLab. Invest., 90: 459-475. (doi: 10.1038/labinvest.2009. 148) 

2 ) Torigoe, D., Asano, A., Yamauchi, H., Dang, R., Sasaki, N. and Agui, T. 2010. Genetic analysis of modifiers for the hooded phenotype in the rat. Jpn. J. Vet. Res.57: 175-184. 

3 ) Iwata, R., Sasaki, N. and Agui, T. 2010. Contiguous gene deletion of Ptprk andThemis causes T-helper immunodeficiency (thid) in the LEC ratBiomed. Res.,31: 83-87. (doi:10.2220/biomedres.31.83)

 4 )Otsuka, S., Namiki, Y., Ichii, O., Sasaki, N., Hashimoto, Y. and Kon, Y. 2010. Analysis of factors decreasing testis weight in MRL miceMamm. Genome21: 153-161. (doi: 10.1007/s00335-010-9251-0)

5 ) Ichii, O., Kamikawa, A., Otsuka, S., Hashimoto, Y., Sasaki, N., Endoh, D. and Kon, Y. 2010.Overexpression of interferon-activated gene 202 (Ifi202) correlates with the progression of autoimmune glomerulonephritis associated with the MRL chromosome 1. Lupus19: 897-905. (doi:10.1177/0961203310362534) 

6 ) Hosoda, Y., Sasaki, N. and Agui, T. 2010. Hypothyroid phenotype of the Tpst2mutant mouse is dependent upon genetic backgroundBiomed. Res., 31: 207-211. (doi:10.2220/biomedres.31.207) 

7 ) Nishino, T., Sasaki, N., Nagasaki, K., Zulkifli, A. and Agui, T. 2010. Genetic background strongly influences the severity of glomerulosclerosis in miceJ. Vet. Med. Sci.,72: 1313-1318. (doi:10.1292/jvms.10-0144) 



Original articles


1 ) Nagasaki, K., Ikoma, T., Katsuda, S., Tonegawa, T., Tanaka, J., Nakamura, T., Sato, H., Ito, S., Sasaki, N., and Agui, T. 2009. Sustained efficacy of erythropoietin with a hydroxyapatite carrier administered in miceJ. Vet. Med. Sci., 71: 729-736.

2 ) Moritoh, K., Yamauchi, H., Asano, A., Yoshii, K., Kariwa, H., Takashima, I., Isoda, N., Sakoda, Y., Kida, H., Sasaki, N., and Agui, T. 2009. Generation of congenic mouse strains by introducing the virus-resistant genes, Mx1 and Oas1b, of feral mouse-derived inbred strain MSM/Ms into the common strain C57BL/6JJpn. J. Vet. Res.57: 89-99.

3 ) Nagasaki, K., Ikoma, T., Katsuda, S., Tonegawa, T., Tanaka, J., Ohyama, M., Hayashida, K., Nakamura, T., Sato, H., Ito, S., Sasaki, N., and Agui, T. 2009. Amelioration of anemia in the ICGN mouse, a renal anemia model, with a subcutaneous bolus Injection of erythropoietin adsorbed to hydroxyapatite matrixJ. Vet. Med. Sci.71: 1365-1371.

4 ) Simon, A.Y., Moritoh, K., Torigoe, D., Asano, A., Sasaki, N., and Agui, T. 2009.Multigenic control of resistance to Sendai virus infection in mice. Infect. Genet. Evol.9: 1253-1259.

5 ) Ichii, O., Konno, A., Sasaki, N., Endoh, D., Hashimoto, Y., and Kon, Y. 2009. Onset of autoimmune glomerulonephritis derived from the telomeric region of MRL-chromosome 1 is associated with the male sex hormone in miceLupus18: 491-500.



Original articles


1 ) Kariwa, H., Noda, H., Nakauchi, M., Ishizuka, M., Hashiguchi, K., Hashimoto, S., Yoshii, K., Asano, A., Agui, T., Kogaki, H., Kurano, Y., Uchida, Y. Fujii, N., Okada, M. and Takashima, I. 2008. Characterization and epitope mapping of monoclonal antibodies to the nucleocapsid protein of severe acute respiratory syndrome coronavirusJpn. J. Vet. Res55: 115-127.

2 ) Hosoda, Y., Sasaki, N., and Agui, T. 2008. Female infertility in grt mice is caused by thyroid hormone deficiency, not by insufficient TPST2 activity in the reproductive organsJ. Vet. Med. Sci70: 1043-1049.

3 ) Ichii, O., Konno, A., Sasaki, N., Endoh, D., Hashimoto, Y. and Kon, Y. 2008. Altered balance of inhibitory and active Fc gamma receptors in murine autoimmune glomerulonephritisKidney Int74: 339-347.

4 ) Ichii, O., Konno, A., Sasaki, N., Endoh, D., Hashimoto, Y. and Kon, Y. 2008. Autoimmune glomerulonephritis induced in congenic mouse strain carrying telomeric region of chromosome 1 derived from MRL/MpJ. Histol. Histopathol23: 411-422.

5 ) Otsuka, S., Konno, A., Sasaki, N., Endoh, D., Hashimoto, Y. and Kon, Y., 2008. Oocytes in newborn MRL mouse testes. Biol. Reprod79: 9-16.

6 ) Otsuka, S., Konno, A., Sasaki, N., Endoh, D., Hashimoto, Y. and Kon, Y., 2008. Polymorphism in MRL and AKR mice Sry: a candidate gene for the appearance of testicular oocyte. Jpn. J. Vet. Res56: 129-138.



Original Articles


1 ) Okumura, M., Yoshimatsu, K., Kumperasart, S., Nakamura, I., Ogino, M., Taruishi, M., Sungdee, A., Pattamadilok, S., Ibrahim, I. N., Erlina, S., Agui, T., Yanagihara, R. and Arikawa, J. 2007. Development of serological assays for Thottapalayam virus, an insectivore -borne Hantavirus. Clin. Vaccine Immunol., 14: 173-181.

2 ) Menon, S., Lee, J., Abplanalp,W. A., Yoo, S.-E., Agui, T., Furudate, S., Kim, P. S. and Arvan, P. 2007. Oxidoreductase interactions include a role for ERp72 engagement with mutant thyroglobulin from the rdw/rdw rat dwarf. J. Biol. Chem., 282: 6183-6191.

3 ) Sasaki, N., Hosoda, Y., Nagata, A., Ding, M., Cheng, J.-M., Miyamoto, T., Okano, S., Asano, A.,Miyoshi, I. and Agui, T. 2007. A mutation in Tpst2 encoding tyrosylprotein sulfotransferase causes dwarfism associated with hypothyroidism. Mol. Endocrinol., 21: 1713-1721.

4 ) Asano, A., Tsubomatsu, K., Jung, C.-G., Sasaki, N. and Agui, T. 2007. A deletion mutation of the protein tyrosine phosphatase kappa (Ptprk) gene is responsible for the T-helper immunodeficiency (thid) in the LEC rat. Mamm. Genome, 18: 779-786.



Original Articles


1 ) Cho, A-R., Uchio-Yamada, K., Torigai, T., Miyamoto, T., Miyoshi, I ., Matsuda, J., Kurosawa, T., Kon, Y., Asano, A., Sasaki, N.and Agui , T. 2006. Deficiency of the tensin2 gene in the ICGN mouse, an animal model for congenital nephrotic syndrome. Mamm. Genome, 17: 407-416.

2 ) Masuda, K., Miyamoto, T., Cho, A-R. and Agui, T. 2006. Analysis of the cell cycle of fibroblasts derived from the LEC rat after X-irradiation. Jpn. J. Vet. Res., 53: 141-148.

3 ) Agui, T. 2006. IV-E1. Use of Laboratory Animals.,  VII-A3 (1). Infectious diseases in livestock and vaccine. In : Handbook of Experimental Immunology. Ed. Izumi Nakashima, The University of Nagoya Press, p170-174, p325 (in Japanese).

4 ) Izawa, M., Kitamura, N., Odake, N., Maki, F., Kanehira, K., Nemoto, H., Yamaguchi, M., Yamashita,A., Sasaki, N., Hattori, M., Kanayama, S. and Yoneda , Y. 2006. A rapid and simple transcriptional sequencing method for GC-rich DNA regions. Jpn. J. Vet. Res., 53: 159-68.



Original Articles

1 ) Namiki, Y., Kon, Y., Kazusa, K., Asano, A., Sasaki, N. andAgui, T. 2005. Quantitative trait loci analysis of heat stress resistance of spermatocytes in the MRL/MpJ mouse. Mamm. Genome, 16: 96-102.

2 ) Okano, S., Asano, A., Sasaki, N., Kon, Y., Watanabe, T. and Agui, T. 2005. Examination of the Lunatic fringe and Uncx 4. 1 expression by whole-mount in situ hybridization in the embryo of the CKH-Jsr (jumbled spine and ribs) mouse. Jpn. J. Vet. Res., 52: 145-149.

3 ) Nakamura, T., Asano, A., Okano, S., Ko, J.‐H., Kon, Y. , Watanabe, T. and Agui , T. 2005. Intracellular localization and their antiviral property of canine Mx proteins. J. Interferon Cyokine Res., 25: 169-173.



Original Articles


1 ) Ko, J-H., Asano, A., Kon, Y. and Agui, T. 2004. Characterization of the chicken PKR:Polymorphism of the geneand antiviral activity against vesicular stomatitis virus. Jpn. J. Vet. Res., 51:123-133.


2 ) Asano, A., Kon, Y. and Agui, T. 2004. The mRNA regulation of porcine double-stranded RNA-activated protein kinase gene. J. Vet. Med. Sci., 66:1523-1528.


3 ) Ko, J-H., Takada, T., Mitsuhashi, T., Agui, T. and Watanabe, T. 2004. Native antiviral specificity of chicken Mxprotein depends on amino acid variation at position 631. Anim. Genet., 35:119-122.


4 ) Okumura, M., Yoshimatsu, K., Araki, K., Lee, B-H., Asano, A., Agui. T. and Arikawa, J. 2004. Epitope analysis of monoclonal antibody E5/G6, which binds to a linear epitope in the nucleocapsid protein of hantaviruses. Arch. Virol., 149: 2427-2434.


5 ) Asano, A., Kon, Y. and Agui, T., 2004. The mRNA regulation of porcine double-stranded RNA-activated protein kinase gene. J. Vet. Med. Sci., 66:1523-1528.


6 ) Namiki, Y., Kon, Y., Sasaki, N., Agui, Y. and Endoh, D. 2004. Exon skipping of exonuclease 1 in MRL/MpJ mice is caused by a nucleotide substitution of the branchpoint sequence in intron eight. Jpn. J. Vet. Res., 52,:125-134.



Original Articles


1 ) Asano, A., Jin, H. K., Watanabe, T. 2003. Mouse Mx2 gene: organization, mRNA expression and the role of the interferon-response promoter in its regulation. Gene 306: 105-113.



Original Articles

1 ) J.-M. Cheng, M. Ding, T. Miyamoto, O. Fujimori, & T. Agui. 2002. Investigation of post-transcriptional events of the thyroglobulin in the thyroid gland of the hypothyroid growth-retarded mouse DW/J-grt. Nagoya Med. J., 45: 133-143.


2 ) Okui, T., Endoh, D., Kon, Y. and Hayashi, M. 2002. Defeciancy in nuclear accumulation of G22p1 and Xrcc5 proteins in hyper-radiosensitive Long-Evans Cinnamon (LEC) rat cells after X irradiation. Rad. Res., 157: 553-561.


3 ) Kitamura, G., Ohta, T., Kai, T., Kon, Y. and Ito, S. 2002. Inhibitory effects of opioids on voltage-dependent Ca2+ channels and catecholamine secretion in cultured porcine adrenal chromaffin cells. Brain Res., 942(1-2): 11-22.


4 ) Asanuma, T., Ishibashi, H., Konno, A., Kon, Y., Inanami, O. and Kuwabara, M. 2002. Assessment of neuroprotective ability of a spin trap, a-phenyl-N-tert-butylnitrone, against malonate-induced ischemic injury of rat brain by apparent water diffusion coefficient mapping. Neurosci. Lett., 329: 281-284.


5 ) Miyoshi, I., Takahashi, K., Kon, Y., Okamura, T., Mototani, Y., Araki, Y. and Kasai, N. 2002. Mouse transgenic for murine oviduct-specific glycoprotein promoter-driven Simian Virus 40 large-T antigen: Tumor formation and its hormonal regulation. Mol. Reprod. Dev., 63: 168-176.


6 ) Kon, Y., Namiki, Y. and Endoh, D. 2002. Expression and distribution of inducible nitric oxide synthase in mouse testis. Jpn. J. Vet. Res., 50(2-3): 2002.


7 ) Asano, A., Ko, J.H.,, Morozumi, T., Hamasima, N. and Watanabe, T. 2002. Polymorphisms and the antiviral property of porcine Mx1 protein. J. Vet. Med. Sci. 64: 1085-1089.


8 ) Ko, J.H., Jin, H.K., Asano, A., Takada, A., Ninomiya, A., Kida, H., Hokiyama, H., Ohara, M., Tsuzuki, M., Nishibori, M., Mizutani, M. and Watanabe, T. 2002. Polymorphisms and the differential antiviral activity of the chicken Mx gene. Genome Res. 12: :595-601.


9 ) Okano S, Asano A, Kon Y, Miyoshi H, Watanabe T, 2002. Genetic analysis of jumbled spine and ribs (Jsr) mutation affecting the vertebral development in mice. Biochem. Genet. 40: 311-322.


Publications other than original articles


1 ) Kon, Y. 2002. General anatomy pp6-10. In: Anatomy of the Dog. An illustrated Text. 2nd Edition. Eds. Hayashi, Y. and Hashimoto, Y., Gauso-sha, Tokyo.



Original Articles


1 ) Asano, A., Irie, Y., Saito, M. 2001. Isoform-specific regulation of vascular endothelial growth factor (VEGF) family mRNA expression in cultured mouse brown adipocytes. Mol. Cell Endocrinol. 174: 71-76.


2 ) Nakamura, Y., Nagase, I., Asano, A., Sasaki, N., Yoshida,T., Umekawa, T., Sakane, N. and Saito M. 2001. β3-adrenergic agonist up-regulates uncoupling proteins 2 and 3 in skeletal muscle of the mouse. J. Vet. Med. Sci. 63: 309-314.


3 ) Jin, H. K., Yoshimatsu, K., Takada, A., Ogino, M., Asano, A., Arikawa, J., Watanabe, T. 2001. Mouse Mx2 protein inhibits hantavirus but not influenza virus replication. Arch. Virol. 146: 41-49.


4 ) Ohuchi, J., Arai, T., Kon, Y., Asano, A., Yamauchi, H. and Watanabe, T. 2001. Characterization of a novel gene, sperm-tail-associated protein (Stap), in mouse post-meiotic testicular germ cells. Mol. Reprod. Dev. 59: 350-358.


5 ) El-Shazly, S., Seo, K. W., El-Nahas, A., Ables, G. P., Asano, A. and Watanabe, T. 2001. Male Hybrid sterility of mice with the genomic region of the KitW mutation and KitS allele from Mus spretus. Biochem. Genet. 39: 127-137.


6 ) Morozumi, T., Sumantri, C., Nakajima, E., Kobayashi, E., Asano, A., Oishi, T., Mitsuhashi, T., Watanabe, T. and Hamasima, N. 2001. Three types of polymorphisms in exon 14 in porcine Mx1 gene. Biochem. Genet. 39: 251-260.


7 ) El-Shazly, S., Okano, S., Asano, A. and Watanabe, T. 2001. Developmental study of the different effects on the hybrid sterility of KitW and KitW-V alleles paired with KitS from Mus spretus. Develop. Growth Differ. 43: 611-617.


8 ) Kon, Y. and Endoh, D. 2001. Heat-shock resistance in experimental cryptorchid testis of mice. Mol. Reprod. Develop. 58: 216-222.


9 ) Endoh, D., Okui, T., Kon, Y. and Hayashi, M. 2001. Hypertonic treatment inhibits radiation-induced neculear translocation of the Ku proteins G22p1 (Ku70) and Xrcc5 (Ku80) in rat fibroblasts. Rad. Res. 155: 320-327.


10) Kiba, T., Saito, S., Numata, K., Kon, Y., Mizutani, T. and Sekihara, H. 2001. Expression of apoptosis on rat liver by hepatic vagus hyperactivity after ventromedial hypothalamic lesioning. Am. J. Physiol: Gastrointestinal and Liver Physiology 280: G958-G967.


11) Asanuma, T., Ohkura, K., Yamamoto, T, Kon, Y., Shimokawa, S. and Kuwabara, M. 2001. Three-dimensional magnetic resonance imaging of lung and liver tumors in mice by use of transversal multislice magnetic resonance images. Comp. Med. 51: 138-144.


12) Mori, K., Kon, Y., Konno, A. and Iwanaga, T. 2001. Cellular Distribution of Napsin (Kidney-derived Aspartic Protease-like Protein, KAP) mRNA in the Kidney, Lung and Lymphatic Organs of Adult and Developing Mice. Arch. Histol. Cytol. 64: 319-327


13) Miyoshi, I., Maki, K., Kon, Y., Yamashita, T., Aoyama, S., Hayashizaki, Y. and Kasai, N. 2001. Targeting oncogenesis by introduction of a 5.2-Kbp segment of the 5’ regulatory region of the human thyrotropin b-subunit gene. Endocrine Res. 27: 387-398.


14) Takada, G., Jin, M. B., Masuko, H., Yamashita, K, Kitagawa, N., Takeda, K., Sakurai, N., Kon, Y., Horiuchi, H., Shimamura, T., Furukawa, H. and Todo, S. 2001. Role of local renin-angiotensin system in warm ischemia and reperfusion injury of the liver. Transplant. Proc. 33: 824-825.



Original Articles


1 ) Yanai, K., Saito, T., Kakinuma, Y., Kon, Y., Hirota, K., Taniguchi-Yanai, K., Nishijo, N., Shigematsu, Y., Horiguchi, H., Kasuya, Y., Sugiyama, F., Yagami, K., Murakami, K. and Fukamizu, A. 2000. Renin-dependent cardiovascular functions and renin-independent blood-brain barrier functions revealed by renin-deficient mice. J. Biol. Chem. 275 : 5-8.


2 ) Seo, K. W., Kon,Y. and Watanabe, T. 2000. Embryonic hematopoiesis defect in the Tattered-Hokkaido (Tdho) mouse. Lab. Anim. Sci, 50: 8-12.


3 ) Okamura, T., Imai, Y. Kon, Y., Goto, M., Yamamoto, M., and Watanabe,T. 2000. Molecular cloning and characterization of mouse testis poly(A) binding proteinⅡencoded by the Pabp3 gene, which transcomplements meiotic mutant sme2 of S. pombe. Biochem. Genet, 38: 1-11.


4 ) Kon, Y. and Endoh, D. 2000. Morphological study of metaphase-specific apoptosis in MRL mouse testis. Anat. Histol. Embryol. 29 : 313-319.


5 ) Endoh, D., Cho, K. O., Tsukamoto, K., Morimura, T., Kon, Y. and Hayashi, M. 2000. Application of representational differential analysis to genomic fragments of marek’s disease virus. J. Clin. Microbiol. 38 : 4310-4314.


6 ) Kimura, K., Sasaki, N., Asano, A., Mizukami, J., Kayahashi, S., Kawada, T., Fushiki, T., Morimatsu, M. and Saito, M. 2000. Mutated human _ 3-adrenergic receptor (Trp 64 Arg) lowers the response to _ 3-adrenergic agonists in transfected 3T3-L preadipocytes. Horm. Metab. Res. 32 : 91-96.


Publications other than original articles


1 ) Inanami, O., Ohta, T., Kon, Y., Hiraoka, W. and Kuwabara, M. 2000. Oxidativestress and signal transduction. Radiol. Sci. 43 : 104-112 (in Japanese)



Original articles


1 ) Miyoshi, M., Kon, Y., Seo, K. W., Jin, H. K., Hasegawa, I. and Watanabe, T. 1999. Jumbled spine and ribs (Jsr) : a new mutation on mouse chromosome 5 . Mammal. Genome, 10 : 213-217.


2 ) Jin, H. K.,Takada, A., Kon, Y., Haller, O. and Watanabe, T. 1999. Identification of the murine Mx 2 gene : interferon-induced expression of the Mx 2 protein from the feral mouse gene confers resistance to vesicular stomatitis virus. J Virol ., 73 : 4925-4930.


3 ) Asanuma, T., Hirano, Y., Yamamoto, K., Kon, Y., Shimokawa, S. and Kuwabara, M. 1999. MR imaging of hepatic injury in the LEC rat under a high magnetic field (7. 05T) . J Vet. Med. Sci ., 61 : 239-244.


4 ) Kon, Y., Horikoshi, H. and Endoh, D. 1999. Metaphase-specific cell death in meiotic spermatocyies in mice. Cell Tissue Res., 296 : 359-369.


5 ) Kon, Y. and Endoh, D. 1999. Expression of angiotensinogen in the uterus induced by coagulating gland renin in mice. Anat. Histol. Embryol ., 28 : 119-124.


6 ) Kon, Y. and Endoh, D. 1999. Renin in exocrine glands of variable mouse strains . Anat. Histol. Embryol ., 28 : 239-242.


7 ) Kblood, E. B. M., Miyoshi, H., Iwala, H., Kazusaka, A., Kon, Y., Hadid, A. H. A., Moustafe, E. K., Ghonim, M. H. and Fijita, S. 1999. Effects of concurrent exposure to 3 -methylcholanthrene and vitamin A on fetal development in rats. Jpn. J Vet. Res.,47 : 13-23.


8 ) Kon, Y., Endoh, D. and lwanaga, T. 1999. Expression of protein gene product 9. 5, a neuronal ubiquitin C-terminal hydrolase, and its developing change in Sertoli cells of mouse testis. Mol. Reprod. Dev., 54 : 333-34 1 .


9 ) Irie, Y., Asano, A., Canas, X., Nikami, H., Aizawa, S. and Saito, M. 1999. Immotal brown adipocyles from p53-knockout mice : differentiation and expression of uncoupling proteins. Biochem. Biophys. Res. Commun ., 255 : 221-225.


10) Asano, A., Kimura, K. and Saito, M. 1999. Cold-induced mRNA expression of angiogenic factors in rat brown adipose tissue. J Vet. Med. Sci .,61 : 403-409.


11) Yanai, K., Saito, T., Kakinuma, Y., Kon, Y., Hirota, K., Taniguchi-Yanai, K., Nishijo, N., Shigematsu, Y., Horiguchi, H., Kasuya, Y., Sugiyama, F., Yagami, K., Murakami, K. and Fukamizu, A. 2000. Renin-dependent cardiovascular functions and renin-independent blood-brain barrier functions revealed by renin-deficient mice. J Biol. Chem.,275 : 5 -8.


12) Seo, K. W., Kon, Y. and Watanabe, T. 2000. Embryonic hematopoiesis defect in the Tattered-hokkaido (Td ho) mouse. Lab. Anim. Sci.,50 : 8 -12.


Publications other than original articles


1 ) Kon, Y. (1999) : Comparative study of renin-containing cells. Histological approaches. J Vet. Med. Sci., 61 : 1075-1 086 .



Original articles


1 ) Endoh, D., Ito, M., Cho, K.-O., Kon, Y., Morimura, T., Hayashi, M. and Kuwabara, M. 1998. Retroviral sequence located in border region of short unique region and short terminal repeat of Md5 strain of Marek's disease virus type1. J. Vet. Med. Sci., 60: 227-235.


2 ) Kon, Y., Endoh, D., Yamashita, T., and Watanabe, T. 1998. Expression of renin in the rat liver. Anat. Histol. Embryol., 27: 111-118.


3 ) Kon, Y., Miyoshi, M. and Hashimoto, Y. 1998. Chemoreceptive epithelioid cells in the chicken aorta. An electron microscopical study. Anat. Histol. Embryol., 27: 161-166.


4 ) Ui, M., Endoh, D., Cho, K.-O., Kon, Y., Iwata, A., Maki, Y., Sato, F. and Kuwabara, M. 1998. Transcriptional analysis of Marek's disease virus (MDV) genes in MDV-transformed lymphoblastoid cell lines without MDV-activated cells. J. Vet. Med. Sci., 60: 823-829.


5 ) Asanuma, T., Shimokawa, S., Inanami, O, Kon, Y. and Kuwabara, M. 1998. Visualization of the topographical structure of the anesthetized mouse brain by MR microimaging. J. Vet. Med. Sci., 60: 1311-1314.


6 ) Jin, H. K., Yamashita, T., Ochiai, K., Haller, O. and Watanabe, T. 1998. Characterization and expression of the Mx1 gene in wild mouse species. Biochem. Genet., 36: 311-322.


7 ) Ishida, J., Asada, S., Daitoku, H., Fujiwara, K., Kon, Y., Sugaya, T., Murakami, K., Nakajima, T., Kasuya, Y. and Fukamizu, A. 1999. Expression and characterization of mouse angiotensin II type 1a receptor tagging hemagglutinin epitope in cultured cells. Int. J. Mol. Med., 3: 263-270.


Publications other than original article


1 ) Hayashi, M., Ishida, K., Maeda, A., Kon, Y., Mizutani, T., Watanabe, T., Arai, S. and Okada, F. 1998. Intramuscular injection of plasmid DNA expressing mRNA7 coding the nucleocapsid protein of JHMV partially protected mice against acute infection with JHMV. In: Advances in Experimental Medicine and Biology, Coronaviruses and Arteriviruses, 440: 693-699, Enjuanes, L., Siddel, S. G. and Spaan, W.eds., Plenum Press, New York.


2 ) Watanabe, T. 1998. Comparative genetics. In: New Laboratory Animal Science, pp. 9-14, Maejima, K. and Kasai. N. eds., Asakura Shoten, Tokyo (in Japanese).



Original articles


1 ) Seo, K.-W., Miyoshi, H., Kon, Y. and Watanabe T. 1997. Chromosomal mapping and developmental study of Tettered-Hokkaido (Tdho). Mammal. Genome, 8 : 578-580.


2 ) Maeda, A., Kon, Y., Watanabe, T. and Hayashi, M. 1997. Resistance to infection with mouse hepatitis virus (MHV) in the cell clones derived from persistently infected DBT cells with the JHM strain of MHV. J. Vet. Med. Sci., 59 : 545-549.


3 ) Kon, Y., Miyoshi, I., Maki, K., Yamashita, T., Aoyama, S., Watanabe, T., Hayashizaki, Y. and Kasai, N. 1997. Morphological study of pituitary tumorigenesis in transgenic mice induced by tryrotropin b-subunit and Simian virus 40 large T-antigen hybrid oncogene. Histol. Histopathol., 12 : 981-990.


4 ) Asanuma, T., Hirano, Y., Ohkura, K., Kon, Y., Shimokawa, S., Kasai, N. and Kuwabara, M. 1997. Magnetic resonance imaging of hepatocellular carcinona in Long-Evans Cinnamon rats under a magnetic field of 7.05 T. Jpn. J. Vet. Res., 45 : 147-151.


5 ) Yamashita, T., Yamazaki, H., Kon, Y., Watanabe, T., Arikawa, J., Miyoshi, I., Kasai, N. and Kuwabara, M. 1997. Progressive effect of a-phenyl-N-tert-butyl nitrone (PBN) on rat embryo development in vitro. Free Rad. Biol. Med., 23 : 1073-1077.



Publications other than original articles


1 ) Morimura T., Ohashi K., Kon Y., Hattori M., Sugimoto C. and Onuma M. 1997. Apoptosis in peripheral CD4+T cells and thymocytes by Marek's disease virus-infection. Leukemia, 11(Suppl 3) : 206-208.



Original articles


1 ) Nagao, S., Watanabe, T., Ogiso, N., Marunouchi, T. and Takahashi, H. 1995. Genetic mapping of the polycystic kidney gene, pcy, on mouse chromosome 9. Biochem. Genetics, 33: 401-412.


2 ) Hayashi, M., Ishimori, K., Maeda, A., Watanabe, T., Arai, S. and Okui, T. 1996. Radioresistant DNA synthesis in fibroblast cell lines derived from LEC strain rats. Mutation Res., 352: 117-121.


3 ) Ismail, H. I., Hashimoto, Y., Kon, Y., Konno, A., Yamazoe, H. and Iwanaga, T. 1996. Immunoglobulin-containing cells and T Iymphocyte subpopulations in the mouse mammary gland. A morphometric and immunohistochemical study. Biomed. Res., 17: 105-113,


4 ) Kahn, M. Z. I., Hashimoto, Y., Konno, A., Kon, Y. and Iwanaga, T. 1996. Development of T-lymphocyte subpopulations in the postnatal chicken oviduct. Ceii Tissue Res., 284: 317-325.


5 ) Ismail, H. I., Hashimoto, Y., Kon, Y., Okada, K., Davis, W. C. and Iwanaga, T. 1996. Lymphocyte subpopulations in the mammary gland of the goat. Vet. Immunol. Immunopathol., 52: 201-212.


6 ) Hayashi, M., Okada, F., Ishida, K., Maeda, A., Kon, Y., Mizutani, T., Itoh, T., Arai, S. and Watanabe, T. 1996. Cytolytic activity induced by intramuscular injection of plasmid DNA expressing the nucleocapsid protein of the JHM strain of mouse hepatitis virus into C57BL / 6 mice. J. Vet. Med Sci., 58: 731-735.


7 ) Lee, J.-H., Hashimoto, Y., Kon, Y., Sugimura, M. and Lee, H.-S. 1996. Immunoelectron microscopic study on the endocrine pancreas of the native Korean goat. Korean J. Electron Microsc., 26: 67-77.


8 ) Yamashita, T., Ohshima, H., Asanuma, T., Inukai, N., Miyoshi, I., Kasai, N., Kon, Y., Watanabe, T., Sato, F. and Kuwabara, M. 1996. The effects of a-phenyl-tert-butylnitrone (PBN) on copper-induced rat fulminant hepatitis with jaundice. Free Radical Biol. Med., 21: 755-761.


9 ) Endoh, D., Kon, Y., Hayashi, M., Morimura, T., Cho, K.-O., Iwasaki, T. and Sato, F. 1996. Detection of transcripts of Marek's disease virus serotype 1 ICP4 homologue (MDV1 ICP4) by in situ hybridization. J. Vet. Med. Sci., 58: 969-975.


10) Nagata, M., Tanimoto, K., Fukamizu, A., Kon, Y., Sugiyama, F., Yagami, K., Murakami, K. and Watanabe, T. 1996. Nephrogenesis and renovascular development in angiotensinogen-deficient mice. Lab. Invest., 75: 745-753.


11) Kon, Y., Endoh, D., Fukamizu, A., Murakami, K., Yamashita, T. and Watanabe, T. 1996. Detection of coagulating gland renin by hybridohistochemistry. Anat. Histol. Embryol , 25: 289-294.


12) Morimura, T., Ohashi, K., Kon, Y., Hattori, M., Sugimoto, C. and Onuma, M. 1996. Apoptosis and CD8-down-regulation in the thymus in chickens infected with Marek's disease virus. Arch. Virol., 141: 2243-2249.


Publication other than original articles


1 ) Kon, Y. and Endoh, D. 1996. Expression of renin in coagulating glands. Histol. Histopathol., 11: 257-266.


2 ) Kon, Y. 1996. The history of renin-producing cells. Gut Intest. Hormones, 14: 1-7 (in japanese).


3 ) Kon, Y. 1996. Local renin-angiotensin system: especially in coagulating glands of mice. Arch. Histol. Cytol. , 59: 399-420.